Performance of "RESIST-3 O.K.N. K-SeT" immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey

Abstract

In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n=22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla_KPC, bla_IMP, bla_VIM, bla_NDM, and bla_OXA-48. The rates of bla_NDM, bla_OXA-48, and bla_KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla_NDM and bla_OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying bla_KPC, bla_NDM, and/or bla_OXA-48. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring bla_IMP and bla_VIM. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.

Keywords: Immunochromatographic test; KPC; Klebsiella pneumoniae; NDM; OXA-48.

Fig. 1

Evaluation of the test results in the RESIST-3 O.K.N.K-SeT assay. 1; KPC producing K. pneumoniae, 2; OXA-48 producing K. pneumoniae, 3; NDM producing K. pneumoniae, 4; OXA-48 and NDM co-producing K. pneumoniae, 5; Carbapenem susceptible K. pneumoniae as negative control, 6; IMP producing E. cloacae, 7; NDM producing E. cloacae, 8; OXA-48 producing S. marcescens, 9; NDM producing E. coli, 10; NDM producing C. freundii, 11; Carbapenem susceptible E. coli as negative control.