Studies of antibody-antigen interactions by capillary electrophoresis: A review

Abstract

Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

Keywords: Affinity capillary electrophoresis; Antibody-antigen interactions; Capillary electrophoresis frontal analysis; Flow through partial filling affinity capillary electrophoresis; Multiple sequential injections of equal volumes; Partial filling affinity capillary electrophoresis.