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. 2018 Apr;36(4):324-327.
doi: 10.1038/nbt.4102. Epub 2018 Mar 19.

Base editing with a Cpf1-cytidine deaminase fusion

Xiaosa Li  1   2   3 , Ying Wang  4 , Yajing Liu  1   2   3 , Bei Yang  5 , Xiao Wang  1   2   3 , Jia Wei  4 , Zongyang Lu  1   2   3 , Yuxi Zhang  1 , Jing Wu  1 , Xingxu Huang  1 , Li Yang  4 , Jia Chen  1
Affiliations

Base editing with a Cpf1-cytidine deaminase fusion

Xiaosa Li et al. Nat Biotechnol. 2018 Apr.

Abstract

The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a CRISPR-Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C-to-T substitutions and off-target editing.

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References

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