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. 2017 Dec 14:22:37-43.
doi: 10.1016/j.nmni.2017.12.005. eCollection 2018 Mar.

'Enterococcus timonensis' sp. nov., 'Actinomyces marseillensis' sp. nov., 'Leptotrichia massiliensis' sp. nov., 'Actinomyces pacaensis' sp. nov., 'Actinomyces oralis' sp. nov., 'Actinomyces culturomici' sp. nov. and 'Gemella massiliensis' sp. nov., new bacterial species isolated from the human respiratory microbiome

Affiliations

'Enterococcus timonensis' sp. nov., 'Actinomyces marseillensis' sp. nov., 'Leptotrichia massiliensis' sp. nov., 'Actinomyces pacaensis' sp. nov., 'Actinomyces oralis' sp. nov., 'Actinomyces culturomici' sp. nov. and 'Gemella massiliensis' sp. nov., new bacterial species isolated from the human respiratory microbiome

M D Mbogning Fonkou et al. New Microbes New Infect. .

Expression of concern in

Abstract

We report the main characteristics of 'Enterococcus timonensis' strain Marseille-P2817T (CSUR P2817), 'Leptotrichia massiliensis' sp. nov., strain Marseille-P3007T (CSUR P3007), 'Actinomyces marseillensis' sp. nov., strain Marseille-P2818T (CSUR P2818), 'Actinomyces pacaensis' sp. nov., strain Marseille-P2985T (CSUR P2985), 'Actinomyces oralis' sp. nov., strain Marseille-P3109T (CSUR P3109), 'Actinomyces culturomici' sp. nov., strain Marseille-P3561T (CSUR P3561) and 'Gemella massiliensis' sp. nov., strain Marseille-P3249T (CSUR P3249) which were isolated from human sputum samples.

Keywords: Actinomyces sp. nov.; Enterococcus timonensis; Gemella massiliensis; Leptotrichia massiliensis.

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Figures

Fig. 1
Fig. 1
Positioning of ‘Enterococcus timonensis’ strain Marseille-P2817 relative to other phylogenetically close neighbours in phylogenetic tree. Sequences of strains involved were aligned by CLUSTALW, and phylogenetic inferences were obtained by MEGA 7.0 software using maximum-likelihood method. Numbers shown at nodes represent percentages of bootstrap values obtained after 500 repeats to generate majority consensus tree. Only bootstrap scores with minimum 90% score were retained. Scale bar indicates 0.5% nucleotide sequence divergence.
Fig. 2
Fig. 2
Positioning of ‘Leptotrichia massiliensis’ strain Marseille-P3007 relative to other phylogenetically close strains in phylogenetic tree. CLUSTALW was used to align sequences, and phylogenetic inferences were generated by MEGA 7.0 software with maximum-likelihood method. Scale bar indicates 1% nucleotide sequence divergence, and numbers at nodes are percentages of 500 bootstrap values obtained in order to generate consensus tree. Only bootstrap scores with minimum 90% score were retained.
Fig. 3
Fig. 3
Positioning of ‘Actinomyces marseillensis’ strain Marseille-P2818 relative to other phylogenetically close strains in phylogenetic tree. CLUSTALW was used to align sequences, and phylogenetic inferences were generated by MEGA 7.0 software with maximum-likelihood method. Scale bar indicates 1% nucleotide sequence divergence, and numbers at nodes are percentages of 500 bootstrap values obtained in order to generate consensus tree. Only bootstrap scores with minimum 90% score were retained.
Fig. 4
Fig. 4
Positioning of ‘Actinomyces pacaensis’ strain Marseille-P2985 relative to other phylogenetically close strains in phylogenetic tree. CLUSTALW was used to align sequences, and phylogenetic inferences were generated by MEGA 7.0 software with maximum-likelihood method. Scale bar indicates 1% nucleotide sequence divergence, and numbers at nodes are percentages of 500 bootstrap values obtained in order to generate consensus tree. Only bootstrap scores with minimum 90% score were retained.
Fig. 5
Fig. 5
Positioning of ‘Actinomyces oralis’ strain Marseille-P3109 relative to other phylogenetically close strains in phylogenetic tree. CLUSTALW was used to align sequences, and phylogenetic inferences were generated by MEGA 7.0 software with maximum-likelihood method. Scale bar indicates 1% nucleotide sequence divergence, and numbers at nodes are percentages of 500 bootstrap values obtained in order to generate consensus tree. Only bootstrap scores with minimum 90% score were retained.
Fig. 6
Fig. 6
Positioning of ‘Actinomyces culturomici’ strain Marseille-P3561 relative to other phylogenetically close strains in phylogenetic tree. CLUSTALW was used to align sequences, and phylogenetic inferences were generated by MEGA 7.0 software with maximum-likelihood method. Scale bar indicates 1% nucleotide sequence divergence, and numbers at nodes are percentages of 500 bootstrap values obtained in order to generate consensus tree. Only bootstrap scores with minimum 90% score were retained.
Fig. 7
Fig. 7
Positioning of ‘Gemella massiliensis’ strain Marseille-P3249 relative to other phylogenetically close strains in phylogenetic tree. CLUSTALW was used to align sequences, and phylogenetic inferences were generated by MEGA 7.0 software with maximum-likelihood method. Scale bar indicates 1% nucleotide sequence divergence, and numbers at nodes are percentages of 500 bootstrap values obtained in order to generate consensus tree. Only bootstrap scores with minimum 90% score were retained.

References

    1. Lagier J.C., Armougom F., Million M., Hugon P., Pagnier I., Robert C. Microbial culturomics: paradigm shift in the human gut microbiome study. Clin Microbiol Infect. 2012;18:1185–1193. - PubMed
    1. Lagier J.C., Hugon P., Khelaifia S., Fournier P.E., La Scola B., Raoult D. The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota. Clin Microbiol Rev. 2015;28:237–264. - PMC - PubMed
    1. Seng P., Drancourt M., Gouriet F., La Scola B., Fournier P.E., Rolain J.M. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis. 2009;49:543–551. - PubMed
    1. Seng P., Abat C., Rolain J.M., Colson P., Lagier J.C., Gouriet F. Identification of rare pathogenic bacteria in a clinical microbiology laboratory: impact of matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol. 2013;51:2182–2194. - PMC - PubMed
    1. Drancourt M., Bollet C., Carlioz A., Martelin R., Gayral J.P., Raoult D. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol. 2000;38:3623–3630. - PMC - PubMed

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