Foliar Exposure of Cu(OH)2 Nanopesticide to Basil ( Ocimum basilicum): Variety-Dependent Copper Translocation and Biochemical Responses

Abstract

In this study, low and high anthocyanin basil ( Ocimum basilicum) varieties (LAV and HAV) were sprayed with 4.8 mg Cu/per pot from Cu(OH)₂ nanowires, Cu(OH)₂ bulk (CuPro), or CuSO₄ and cultivated for 45 days. In both varieties, significantly higher Cu was determined in leaves of CuSO₄ exposed plants (691 and 672.6 mg/kg for LAV and HAV, respectively); however, only in roots of HAV, Cu was higher, compared to control ( p ≤ 0.05). Nanowires increased n-decanoic, dodecanoic, octanoic, and nonanoic acids in LAV, but reduced n-decanoic, dodecanoic, octanoic, and tetradecanoic acids in HAV, compared with control. In HAV, all compounds reduced eugenol (87%), 2-methylundecanal (71%), and anthocyanin (3%) ( p ≤ 0.05). In addition, in all plant tissues, of both varieties, nanowires and CuSO₄ reduced Mn, while CuPro increased chlorophyll contents, compared with controls ( p ≤ 0.05). Results suggest that the effects of Cu(OH)₂ pesticides are variety- and compound-dependent.

Keywords: Cu(OH)2; anthocyanin; basil; essential oils; fatty acids; nanopesticide; varieties.