Nucleotide sequence of the phosphofructokinase gene from Bacillus stearothermophilus and comparison with the homologous Escherichia coli gene
- PMID: 2956156
- DOI: 10.1016/0378-1119(87)90348-9
Nucleotide sequence of the phosphofructokinase gene from Bacillus stearothermophilus and comparison with the homologous Escherichia coli gene
Abstract
The gene (Bs-pfk) for phosphofructokinase (PFK) from Bacillus stearothermophilus has been cloned and sequenced. The deduced amino acid sequence is nearly identical to the sequence which was previously determined by peptide analysis. The elevated G + C content of Bs-pfk relative to the homologous Ec-pfkA from Escherichia coli is consistent with previous observations concerning genes from thermophilic prokaryotes. A significant degree of homology exists when the deduced amino acid sequence of B. stearothermophilus PFK is compared with the corrected sequences of rabbit muscle PFK or E. coli PFK-1. The cloning and sequencing of Bs-pfk completes the first step toward using site-specific mutagenesis to investigate the structure-function relationships for this allosteric enzyme.
Similar articles
-
The rabbit muscle phosphofructokinase gene: cDNA cloning and sequencing.Proc Natl Sci Counc Repub China B. 1990 Apr;14(2):69-74. Proc Natl Sci Counc Repub China B. 1990. PMID: 2147292
-
High-level expression of Bacillus stearothermophilus 6-phosphofructo-1-kinase in Escherichia coli.Gene. 1987;59(2-3):279-83. doi: 10.1016/0378-1119(87)90335-0. Gene. 1987. PMID: 2963782
-
Evolution of phosphofructokinase--gene duplication and creation of new effector sites.Nature. 1984 May 31-Jun 6;309(5967):467-9. doi: 10.1038/309467a0. Nature. 1984. PMID: 6233492
-
Phosphofructokinase deficiency: recent advances in molecular biology.Muscle Nerve Suppl. 1995;3:S28-34. doi: 10.1002/mus.880181408. Muscle Nerve Suppl. 1995. PMID: 7603524 Review.
-
Regulatory properties and the development of structure in phosphofructokinase.Arch Biol Med Exp. 1987;20(3-4):359-70. Arch Biol Med Exp. 1987. PMID: 8816076 Review. No abstract available.
Cited by
-
Modelling the 2-kinase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase on adenylate kinase.Biochem J. 1997 Feb 1;321 ( Pt 3)(Pt 3):615-21. doi: 10.1042/bj3210615. Biochem J. 1997. PMID: 9032445 Free PMC article.
-
Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus.J Bacteriol. 1993 Sep;175(17):5344-9. doi: 10.1128/jb.175.17.5344-5349.1993. J Bacteriol. 1993. PMID: 8366023 Free PMC article.
-
Enzymes of glucose and methanol metabolism in the actinomycete Amycolatopsis methanolica.J Bacteriol. 1994 Nov;176(22):6827-35. doi: 10.1128/jb.176.22.6827-6835.1994. J Bacteriol. 1994. PMID: 7961441 Free PMC article.
-
In Vivo Ribosome-Amplified MetaBOlism, RAMBO, Effect Observed by Real Time Pulse Chase, RTPC, NMR Spectroscopy.Biochemistry. 2025 Jun 17;64(12):2660-2678. doi: 10.1021/acs.biochem.5c00086. Epub 2025 May 27. Biochemistry. 2025. PMID: 40420686 Free PMC article.
-
Nucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli.J Bacteriol. 1991 May;173(10):3117-27. doi: 10.1128/jb.173.10.3117-3127.1991. J Bacteriol. 1991. PMID: 1850730 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources