Histochemical localization of 3 beta-hydroxysteroid dehydrogenase in marmoset ovaries during pro- and diestrus, with special reference to substrate specificity

Abstract

We applied qualitative cytochemical procedures to investigate and compare the distribution of 3 beta-hydroxysteroid dehydrogenases (HSDH) in pro- and diestrus ovaries of sexually mature marmosets (Callithrix jacchus) using dehydroepiandrosterone or etiocholane-3 beta-ol-17-one as the substrate. During proestrus dehydroepiandrosterone dehydrogenase (3 beta-5 alpha-HSDH) activity was found in the theca of tertiary follicles and in atretic granulosa cells. In granulosa cells at advanced stages of degeneration, HSDH activity was distinctly higher than in thecal cells. The activity of etiocholane-3 beta-ol-17-one dehydrogenase (3 beta-5 beta-HSDH) exhibited a gradient in preovulatory follicles, ranging from high levels in granulosa cells adjacent to the basement membrane to low levels in cells bordering on the antrum and in cumulus oophorus cells. During diestrus 3 beta-5 alpha-HSDH activity was only detected in the corpora lutea; the level of 3 beta-5 beta-HSDH activity was unchanged in the theca of tertiary follicles and was high in the cells of the corpora lutea. HSDH activity was no longer detectable in atretic granulosa cells using either dehydroepiandrosterone or etiocholane-3 beta-ol-17-one as the substrate. Comparison of the distribution of HSDH during proestrus and diestrus revealed that steroidogenesis in marmoset ovaries occurs in follicular elements during diestrus and almost exclusively in the corpora lutea during diestrus. From this phase-dependent localization, it is possible to determine the stage of the estrous cycle. Furthermore, our findings indicate that the localization of HSDH is dependent on the conformational structure of the substrate used.