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. 2017;1(1):15.
doi: 10.1186/s41181-016-0018-0. Epub 2016 Oct 10.

Automation of the Radiosynthesis of Six Different 18F-labeled radiotracers on the AllinOne

Shihong Li  1 , Alexander Schmitz  1 , Hsiaoju Lee  1 , Robert H Mach  1
Affiliations

Automation of the Radiosynthesis of Six Different 18F-labeled radiotracers on the AllinOne

Shihong Li et al. EJNMMI Radiopharm Chem. 2017.

Erratum in

Abstract

Background: Fast implementation of positron emission tomography (PET) into clinical and preclinical studies highly demands automated synthesis for the preparation of PET radiopharmaceuticals in a safe and reproducible manner. The aim of this study was to develop automated synthesis methods for these six 18F-labeled radiopharmaceuticals produced on a routine basis at the University of Pennsylvania using the AllinOne synthesis module.

Results: The development of automated syntheses with varying complexity was accomplished including HPLC purification, SPE procedures and final formulation with sterile filtration. The six radiopharmaceuticals were obtained in high yield and high specific activity with full automation on the AllinOne synthesis module under current good manufacturing practice (cGMP) guidelines.

Conclusion: The study demonstrates the versatility of this synthesis module for the preparation of a wide variety of 18F-labeled radiopharmaceuticals for PET imaging studies.

Keywords: AllinOne; Automation; F-Gln; FTP; FTT; HPLC; ISO-1; PET; Radiotracer; SPE.

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Figures

Fig. 1
Fig. 1
Structures of six radiotracers synthesized on the AllinOne module
Fig. 2
Fig. 2
AllinOne synthesis module with mounted disposable kits and reagents for [18F]FGln
Fig. 3
Fig. 3
AllinOne user software interface for one-vessel reaction ([18F]ISO-1)
Fig. 4
Fig. 4
AllinOne user software interface for two-vessel reaction ([18F]F-Gln)
Fig. 5
Fig. 5
Scheme for the radiosyntheses of the six radiotracers
Fig. 6
Fig. 6
Radioactivity trending of [18F]ISO-1
Fig. 7
Fig. 7
Radioactivity trending of [18F]F-Gln
Fig. 8
Fig. 8
A view of typical semi-preparative chromatogram of [18F]fubatine
Fig. 9
Fig. 9
A view of typical semi-preparative chromatogram of [18F]fallypride
Fig. 10
Fig. 10
A view of typical semi-preparative chromatogram of [18F]FTT
Fig. 11
Fig. 11
A view of typical semi-preparative chromatogram of [18F]FTP
Fig. 12
Fig. 12
A view of typical semi-preparative chromatogram of [18F]F-Gln
Fig. 13
Fig. 13
A view of typical semi-preparative chromatogram of [18F]ISO-1
Fig. 14
Fig. 14
A view of typical analytical chromatogram of [18F]ISO-1
Fig. 15
Fig. 15
Analytical Radio-HPLC chromatography of [18F]F-Gln with different chiral columns
See this image and copyright information in PMC

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