Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jun;208(1-2):10-4.
doi: 10.1007/BF00330415.

Partial purification of an activity from human cells that promotes homologous pairing and the formation of heteroduplex DNA in the presence of ATP

E Cassuto, L A Lightfoot, P Howard-Flanders

Partial purification of an activity from human cells that promotes homologous pairing and the formation of heteroduplex DNA in the presence of ATP

E Cassuto et al. Mol Gen Genet. 1987 Jun.

Abstract

An activity that can promote homologous pairing and strand transfer between suitable DNA substrates has been partially purified from human skin fibroblasts and from HeLa cells. The strand transfer reaction was investigated with DNA substrates consisting of single-stranded circular and duplex linear phage DNA. It requires ATP, and under optimal conditions yields heteroduplex molecules containing one strand from each parental DNA substrate. The reactions appears to be of the same general nature as those mediated by RecA proteins of Escherichia coli and the Rec1 protein of Ustilago maydis.

PubMed Disclaimer

References

    1. Cell. 1983 Jul;33(3):857-64 - PubMed
    1. Nucleic Acids Res. 1984 Apr 11;12(7):3057-68 - PubMed
    1. Cell. 1982 Jun;29(2):367-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4786-90 - PubMed
    1. Cell. 1986 Mar 28;44(6):885-94 - PubMed

Publication types

LinkOut - more resources