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Case Reports
. 2018 Mar 22;12(3):e0006244.
doi: 10.1371/journal.pntd.0006244. eCollection 2018 Mar.

First case of human infection with Plasmodium knowlesi in Laos

Moritoshi Iwagami  1   2   3 , Masami Nakatsu  1   2 , Phonepadith Khattignavong  2   3 , Pheovaly Soundala  2   3 , Lavy Lorphachan  2   3 , Sengdeuane Keomalaphet  2   3 , Phonepadith Xangsayalath  2   3   4 , Satoru Kawai  2   3   5 , Bouasy Hongvanthong  2   6 , Paul T Brey  2   7 , Shigeyuki Kano  1   2   3
Affiliations
Case Reports

First case of human infection with Plasmodium knowlesi in Laos

Moritoshi Iwagami et al. PLoS Negl Trop Dis. .
No abstract available

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Phylogenetic analyses of a Plasmodium knowlesi isolate collected from a malaria patient in the south of the Lao PDR.
(A) The phylogenetic tree of P. knowlesi and other malaria parasites inferred using partial msp1 DNA sequences. (B) The phylogenetic tree of P. knowlesi and other malaria parasites inferred using partial cytb DNA sequences. The analysis was conducted using MEGA software version 7.0.21 [2] (http://www.megasoftware.net) by the neighbor-joining algorithm with Kimura’s 2-parameter model. The bootstrap values from 1,000 replicates are shown at node. Scale bars represent number of nucleotide substitutions per site. GenBank accession numbers and country of origin are shown in related sequences. “No. 947 Lao PDR” indicates the sample sequenced in this study. For the msp1 tree, sequences of P. fieldi, P. coatneyi, P. cynomolgi, P. vivax, P. malariae, and P. falciparum were used as the out-group of the analysis. For the cytb tree, sequences of P. frangile, P. coatneyi, P. cynomolgi, P. vivax, P. malariae, and P. falciparum were used as the out-group of the analysis. The GenBank accession number and country of origin with no species name indicates the sequence of P. knowlesi. DNA sequences of the partial cytb and msp1 were deposited in the DDBJ, for which accession numbers were LC327233 and LC327234, respectively. cytb, cytochrome b gene; DDBJ, DNA Data Bank of Japan; MEGA, molecular evolutionary genetics analysis; msp1, merozoite surface protein-1 gene.
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