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Comparative Study
. 2018 Apr;26(2):545-556.
doi: 10.1016/j.jfda.2017.05.006. Epub 2017 Jun 13.

Modifications of Atlantic salmon by-product oil for obtaining different ω-3 polyunsaturated fatty acids concentrates: An approach to comparative analysis

Monjurul Haq  1 , Seul-Ki Park  2 , Min-Jung Kim  2 , Yeon-Jin Cho  1 , Byung-Soo Chun  1
Affiliations
Comparative Study

Modifications of Atlantic salmon by-product oil for obtaining different ω-3 polyunsaturated fatty acids concentrates: An approach to comparative analysis

Monjurul Haq et al. J Food Drug Anal. 2018 Apr.

Abstract

Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) rich 2-monoacylglycerols (2-MAG), omega-3 polyunsaturated free fatty acids (ω-3 PUFFAs) concentrate, and PUFA enriched acylglycerols were prepared from salmon frame bone oil (SFBO) by enzymatic alcoholysis, urea complexation, and enzymatic esterification, respectively. The yields of 2-MAG, ω-3 PUFFAs concentrate, and PUFA enriched acylglycerols were 40.25, 16.52, and 15.65%, respectively. ω-3 PUFFAs concentrate and PUFA enriched acylglycerols showed darker red color than SFBO and 2-MAG due to aggregation of astaxanthin pigment in ω-3 PUFFAs concentrate during urea complexation. The viscosity and specific gravity of SFBO and PUFA enriched acylglycerols showed similar values whereas 2-MAG and ω-3 PUFFAs showed significantly (p < 0.05) lower values. Stability parameters like acid value, peroxide value, free fatty acid value, and p-anisidine value of SFBO and ω-3 PUFAs concentrates were within acceptable limits except extreme high acid value and free fatty acid value of ω-3 PUFFAs concentrate. Thermogravimetric analysis showed similar and higher thermal stability of SFBO and PUFA enriched acylglycerols than 2-MAG and ω-3 PUFFAs concentrate. The ω-3 PUFAs content in 2-MAG, ω-3 PUFFAs concentrate, and PUFA enriched acylglycerols was increased to 20.81, 52.96, and 51.74% respectively from 13.54% in SFBO. ω-3 PUFFAs concentrate and PUFA enriched acylglycerols showed higher DPPH and ABTS radical scavenging activity than SFBO and 2-MAG. The results obtained from this study suggest the production of PUFA enriched acylglycerols rich in ω-3 PUFAs supplements from fish oil for human and pet animals.

Keywords: 2-MAG; Comparative analysis; PUFA enriched acylglycerols; Salmon frame bone oil; ω-3 PUFFAs concentrate.

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Figures

Fig. 1
Fig. 1
Standard curve used for the determination of astaxanthin content.
Fig. 2
Fig. 2
TLC analysis of SFBO and ω-3 PUFAs concentrates.
Fig. 3
Fig. 3
Yield of different ω-3 PUFAs concentrates. Values are the mean of 3 determinations. Different letters with the bar indicate significant differences (p < 0.05).
Fig. 4
Fig. 4
The pattern of the thermal degradation of SFBO and different ω-3 PUFAs concentrates.
Fig. 5
Fig. 5
Antioxidant activity of SFBO and different ω-3 PUFAs concentrates (a) DPPH radical scavenging activity (b) ABTS+ inhibition activity.
See this image and copyright information in PMC

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