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. 2018 Apr;26(2):788-795.
doi: 10.1016/j.jfda.2017.04.003. Epub 2017 Apr 28.

Simultaneous quantification of eight organic acid components in Artemisia capillaris Thunb (Yinchen) extract using high-performance liquid chromatography coupled with diode array detection and high-resolution mass spectrometry

Fangjun Yu  1 , Hao Qian  1 , Jiayu Zhang  2 , Jie Sun  1 , Zhiguo Ma  1
Affiliations

Simultaneous quantification of eight organic acid components in Artemisia capillaris Thunb (Yinchen) extract using high-performance liquid chromatography coupled with diode array detection and high-resolution mass spectrometry

Fangjun Yu et al. J Food Drug Anal. 2018 Apr.

Abstract

We aim to determine the chemical constituents of Yinchen extract and Yinchen herbs using high-performance liquid chromatography coupled with diode array detection and high-resolution mass spectrometry. The method was developed to analyze of eight organic acid components of Yinchen extract (including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid). The separation was conducted using an Agilent TC-C18 column with acetonitrile - 0.2% formic acid solution as the mobile phases under gradient elution. The analytical method was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery, and subsequently the method was performed for the quantitative assessment of Yinchen extracts and Yinchen herbs. In addition, the changes of selected markers were studied when Yinchen herbs decocting in water and isomerization occurred between the chlorogenic acids. The proposed method enables both qualitative and quantitative analyses and could be developed as a new tool for the quality evaluation of Yinchen extract and Yinchen herbs. The changes of selected markers in water decoction process could give us some novel idea when studying the link between substances and drug efficacy.

Keywords: Artemisia capillaris Thunb (Yinchen) extract; High-performance liquid chromatography; Organic acid; Quality control; Transformation pathways.

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Conflict of interest statement

Conflicts of interest

All authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1
Structures of eight organic acid components.
Fig. 2
Fig. 2
Representative HPLC chromatograms of mixed standards (A), Yinchen herb (B), Yinchen extract (C). The peak numbers are stand for neochlorogenic acid(1), chlorogenic acid (2), cryptochlorogenic acid (3), caffeic acid (4), 1,3-dicaffeoylquinic acid (5), 3,4-dicaffeoylquinic acid (6), 3,5-dicaffeoylquinic acid (7) and 4,5-dicaffeoylquinic acid (8).
Fig. 3
Fig. 3
The change curves of selected markers in water decoction process (A, B).
See this image and copyright information in PMC

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