The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation

doi:10.3390/dj3020024

. 2015 Mar 31;3(2):24-42.

doi: 10.3390/dj3020024.

The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation

Meike Luise Jakobi¹, Sascha Nico Stumpp², Meike Stiesch³, Jörg Eberhard⁴, Wieland Heuer⁵

Affiliations

¹ Private Dental Practice, Hildesheim 31134, Germany. jakobi.meike@gmx.de.
² Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. stumpp.nico@mh-hannover.de.
³ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. stiesch.meike@mh-hannover.de.
⁴ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. eberhard.joerg@mh-hannover.de.
⁵ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. heuer.wieland@mh-hannover.de.

PMID: 29567923
PMCID: PMC5851201
DOI: 10.3390/dj3020024

The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation

Meike Luise Jakobi et al. Dent J (Basel). 2015.

. 2015 Mar 31;3(2):24-42.

doi: 10.3390/dj3020024.

Authors

Meike Luise Jakobi¹, Sascha Nico Stumpp², Meike Stiesch³, Jörg Eberhard⁴, Wieland Heuer⁵

Affiliations

¹ Private Dental Practice, Hildesheim 31134, Germany. jakobi.meike@gmx.de.
² Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. stumpp.nico@mh-hannover.de.
³ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. stiesch.meike@mh-hannover.de.
⁴ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. eberhard.joerg@mh-hannover.de.
⁵ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. heuer.wieland@mh-hannover.de.

PMID: 29567923
PMCID: PMC5851201
DOI: 10.3390/dj3020024

Abstract

Late implant failures, caused by the inflammation of surrounding tissues are a problem in implant dentistry. The path of bacterial transmission from teeth to implants is not completely understood. Therefore, the purpose of this study was to analyze intraindividual bacterial transmission characterizing subgingival microbiomes in teeth and implants, both in healthy subjects and in those with signs of periodontitis or peri-implantitis. Samples of peri-implant and dental sulcus fluid were collected. To identify the predominant microbiota, amplified fragments of bacterial 16S rRNA gene were separated by single strand conformation polymorphism analysis, sequenced and taxonomically classified. A total of 25 different predominant genera were found in the diseased group and 14 genera in the healthy group. Species richness did not differ significantly between implants, neighboring teeth and teeth with largest probing depth in the diseased group. Additionally, no differences between teeth and implants in the healthy group were detected. In contrast, microbial diversity varied between the different sampling points. Species richness is similar in healthy and diseased sites, but the composition of the bacterial community differed within the individual subjects. The underlying analyses strongly suggest that complete transmission from neighboring teeth to implants is unlikely.

Keywords: bacterial biofilms; dental implants; microbial diversity; peri-implantitis; single strand conformation polymorphism/SSCP.

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Conflict of interest statement

The authors declare no conflict of interest.

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References

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[1] Manor Y., Oubaid S., Mardinger O., Chaushu G., Nissan J. Characteristics of early versus late implant failure: A retrospective study. J. Oral Maxillofac. Surg. 2009;67:2649–2652. doi: 10.1016/j.joms.2009.07.050. - DOI - PubMed

[2] Manor Y., Oubaid S., Mardinger O., Chaushu G., Nissan J. Characteristics of early versus late implant failure: A retrospective study. J. Oral Maxillofac. Surg. 2009;67:2649–2652. doi: 10.1016/j.joms.2009.07.050. - DOI - PubMed

[3] Dewhirst F.E., Chen T., Izard J., Paster B.J., Tanner A.C., Yu W.H., Lakshmanan A., Wade W.G. The human oral microbiome. J. Bacteriol. 2010;192:5002–5017. doi: 10.1128/JB.00542-10. - DOI - PMC - PubMed

[4] Dewhirst F.E., Chen T., Izard J., Paster B.J., Tanner A.C., Yu W.H., Lakshmanan A., Wade W.G. The human oral microbiome. J. Bacteriol. 2010;192:5002–5017. doi: 10.1128/JB.00542-10. - DOI - PMC - PubMed

[5] Quirynen M., Vogels R. Clinical relevance of surface characteristics on the formation of plaque on teeth and implants. Ned. Tijdschr. Tandheelkd. 2002;109:422–429. - PubMed

[6] Quirynen M., Vogels R. Clinical relevance of surface characteristics on the formation of plaque on teeth and implants. Ned. Tijdschr. Tandheelkd. 2002;109:422–429. - PubMed

[7] Li J., Helmerhorst E.J., Leone C.W., Troxler R.F., Yaskell T., Haffajee A.D., Socransky S.S., Oppenheim F.G. Identification of early microbial colonizers in human dental biofilm. J. Appl. Microbiol. 2004;97:1311–1318. doi: 10.1111/j.1365-2672.2004.02420.x. - DOI - PubMed

[8] Li J., Helmerhorst E.J., Leone C.W., Troxler R.F., Yaskell T., Haffajee A.D., Socransky S.S., Oppenheim F.G. Identification of early microbial colonizers in human dental biofilm. J. Appl. Microbiol. 2004;97:1311–1318. doi: 10.1111/j.1365-2672.2004.02420.x. - DOI - PubMed

[9] Leonhardt Å., Renvert S., Dahlen G. Microbial findings at failing implants. Clin. Oral Implants Res. 1999;10:339–345. - PubMed

[10] Leonhardt Å., Renvert S., Dahlen G. Microbial findings at failing implants. Clin. Oral Implants Res. 1999;10:339–345. - PubMed

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The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation

Affiliations

The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation

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Conflict of interest statement

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