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. 2018 Feb 8:2018:4761925.
doi: 10.1155/2018/4761925. eCollection 2018.

Effects of Qingshen Granules on the Oxidative Stress-NF/kB Signal Pathway in Unilateral Ureteral Obstruction Rats

Hua Jin  1 , Yiping Wang  1 , Dong Wang  1 , Lei Zhang  1
Affiliations

Effects of Qingshen Granules on the Oxidative Stress-NF/kB Signal Pathway in Unilateral Ureteral Obstruction Rats

Hua Jin et al. Evid Based Complement Alternat Med. .

Abstract

Background. The activation of NF-kappa B (NF/kB) signaling pathway plays an important role in the process of epithelial-mesenchymal transition (EMT) and renal interstitial fibrosis (RIF) in renal tubules. The process of oxidative stress reaction in kidney is via excessive reactive oxygen species (ROS) production to activate NF/kB signaling pathway. Qingshen Granule (QSG) is an effective Chinese formula utilized to treat chronic renal failure. Previous studies confirmed that QSG could inhibit RIF in unilateral ureteral obstruction (UUO) rats. In this study, we used UUO rats to investigate the effects of QSG on oxidative stress and the activation of NF/kB signaling. Seventy male Sprague-Dawley (SD) rats were randomly divided into a sham group, UUO model group, Qingshen Granules (QSG) high-dose, medium-dose, and low-dose groups, PDTC group, and candesartan group (10 rats in each group). Our study demonstrated that oxidative stress-NF/kB signal pathway contributed to the formation of UUO renal interstitial fibrosis. QSG may protect against RIF by inhibiting the oxidative stress-NF/kB signal pathway, reducing inflammation, and improving renal tubular EMT.

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Figures

Figure 1
Figure 1
Comparison of serum creatinine levels with levels of ROS, SOD, and MDA in each group. Notes. ∗ P < 0.01, compared with the sham group; # P < 0.01, ## P < 0.05, compared with the model group; & P < 0.05, compared with the PDTC and candesartan groups.
Figure 2
Figure 2
Expression of ICAM-1, MCP-1, NF-KBp65, p-IKBα, and IKKα in each group. Notes. (a) Original strip chart of Western blot results; (b) relative expression quantity of each protein; ∗ P < 0.01, compared with the sham group; # P < 0.01, ## P < 0.05, compared with the model group; & P < 0.05, compared with the QSG medium-dose group.
Figure 3
Figure 3
Comparison of immunohistochemistry and average optical density of E-cadherin and α-SMA protein in each group. Notes. (a) Immunohistochemistry of E-cadherin protein; (b) immunohistochemistry of α-SMA protein. ∗ P < 0.01, compared with the sham group; # P < 0.01, compared with the model group; & P < 0.01, && P < 0.05, compared with the QSG medium-dose group. (×400).
Figure 4
Figure 4
HE and Masson's stain in each group ((a) HE, (b) Masson ×400). Notes. ∗ P < 0.01, compared with the sham group; # P < 0.01, compared with the model group; & P < 0.01, && P < 0.05, compared with the QSG medium-dose group.
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