Biophysical characteristics of hematopoietic cells during division

Abstract

Cell division is managed by a complex and coordinated sequence of cytoskeleton alterations that give rise to major morphological changes. During dividing the cleavage furrow of the cell is significantly stiffened due to the accumulation of actomyosin. However, it is unclear whether the stiffness on top of the cell is changed or not. Here, we used atomic force microscopy to measure stiffness on this location of non-adhesion Jurkat T cell and its derivative D1.1 cell from interphase to cytokinesis. The results showed that during division the cell stiffness significantly increases at anaphase and telophase. These increases in cell stiffness are most likely due to the cell surface tension created by the pulling forces of the microtubules to separate sister chromatids in the anaphase and the contraction forces of the contractile ring to separate the mother cell into daughters in the telophase. The dynamic measurement of cell elasticity during cell division may be used as a tool to gain further insight into the involved molecules and mechanisms.

Keywords: Cell division; Cell stiffness; Hematopoietic cells; Nanoindentation.