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. 1987;181(3):287-93.

[Giardia intestinalis: influence of the composition of the osmolarity of the medium on in vitro excystation]

[Article in French]
C Chochillon  1 , L A Buchel, J G Gobert, J Savel
Affiliations
  • PMID: 2958118

[Giardia intestinalis: influence of the composition of the osmolarity of the medium on in vitro excystation]

[Article in French]
C Chochillon et al. C R Seances Soc Biol Fil. 1987.

Abstract

The in vitro excystation of Giardia intestinalis was studied to make the osmolarity (from 50 to 500 mosmol/l) and the components of growth medium (MCI saline solution, MCII glucose solution, MCIII nutritive solution) varying. The percentage of excystation, the viability and the generation time were determined. Excystation was observed in the saline solution between 100 to 450 mosmol/l after cyst acid pepsin incubation. The trophozoite viability was increased by glucose addition (60 min in MCI; 300 min in MCII). Only a rich medium (MCIII) permitted a generation time from 225 to 425 mosmol/l.

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