Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Observational Study
. 2018 Mar 27;18(1):51.
doi: 10.1186/s12903-018-0512-8.

Matrix metalloproteinase-8 levels in oral samples as a biomarker for periodontitis in the Chinese population: an observational study

Chao Yuan  1 , Xiaochen Liu  1 , Shuguo Zheng  2
Affiliations
Observational Study

Matrix metalloproteinase-8 levels in oral samples as a biomarker for periodontitis in the Chinese population: an observational study

Chao Yuan et al. BMC Oral Health. .

Abstract

Background: Clinical evaluation of periodontal inflammation does not fully reflect the disease activity. Extensive studies have been conducted out on gingival crevicular fluid (GCF) components that might serve as potential diagnostic markers for periodontitis, among which matrix metalloproteinase-8 (MMP-8) has shown to be promising, but there were no studies for individuals in China. The aim of this study was to compare clinical diagnostic parameters and levels of active MMP-8 (aMMP-8) in GCF and oral rinse samples from the Chinese patients with varying degrees of periodontal inflammation.

Methods: GCF and oral rinse samples were obtained from 60 participants into two groups, a periodontitis group and a control group, specified by the presence and number of pocket depths or attachment loss. The aMMP-8 levels in GCF and oral rinse samples was quantified by ELISA using specific monoclonal antibodies. Logistic and linear regression models were employed for testing the correlation between aMMP-8 levels and periodontal condition, as well as diagnostic sensitivity and specificity.

Results: Periodontitis group (mean = 24.84 ng/ml) exhibited significantly higher aMMP-8 levels than control group in GCF (p < 0.001). The aMMP-8 levels in oral rinse samples ranged from 0.05 to 2.18 ng/ml, but differences were not statistically significant between the two groups (p > 0.1). Receiver operating characteristic curve analysis showed a highest threshold of 6.66, with a corresponding sensitivity and specificity of 0.8 and 0.9, respectively.

Conclusions: Measuring aMMP-8 levels in GCF may have potentiality for complementary early diagnosis of periodontal disease and inflammation in the Chinese population.

Keywords: Diagnostic biomarker; Matrix metalloproteinase-8; Periodontitis.

PubMed Disclaimer

Conflict of interest statement

Ethics approval and consent to participate

The project was approved by the Human Research Ethics Committee of the School of Stomatology, Peking University, China ((No. PKUSSIRB-2012055). Written informed consent for participation was obtained from each subject recruited in this study.

Consent for publication

This manuscript was not involved with details, images, or videos relating to individual participants. Written informed consent of this research was obtained from all the subjects involved.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. McCulloch CA. Collagenolytic enzymes in gingival crevicular fluid as diagnostic indicators of periodontitis. Ann N Y Acad Sci. 1994;732:152–164. doi: 10.1111/j.1749-6632.1994.tb24732.x. - DOI - PubMed
    1. McCulloch CA. Host enzymes in gingival crevicular fluid as diagnostic indicators of periodontitis. J Clin Periodont. 1994;21:497–506. doi: 10.1111/j.1600-051X.1994.tb00414.x. - DOI - PubMed
    1. Kinane DF. Regulators of tissue destruction and homeostasis as diagnostic aids in periodontology. Periodontol2000. 2000;24:215–225. doi: 10.1034/j.1600-0757.2000.2240110.x. - DOI - PubMed
    1. Srinivasan M, Blackburn C, Mohamed M, Sivagami AV, Blum J. Literature-based discovery of salivary biomarkers for type 2 diabetes mellitus. Biomark Insights. 2015;10:39–45. doi: 10.4137/BMI.S22177. - DOI - PMC - PubMed
    1. Reynolds JJ, Hembry RM, Meikle MC. Connective tissue degradation in health and periodontal disease and the roles of matrix metalloproteinases and their natural inhibitors. Adv Dent Res. 1994;8(2):312–319. doi: 10.1177/08959374940080022701. - DOI - PubMed

Publication types

LinkOut - more resources