Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Dec 5;185(1):13.
doi: 10.1007/s00604-017-2559-7.

Microbead-based simultaneous fluorometric detection of three nut allergens

Spyridoula Christopoulou  1 , Sofia Karaiskou  1 , Despina P Kalogianni  2
Affiliations

Microbead-based simultaneous fluorometric detection of three nut allergens

Spyridoula Christopoulou et al. Mikrochim Acta. .

Abstract

A microbead-based method is developed for the detection of three nut allergens that can occur in food. The bead array constitutes of a suspension of spectrally distinct fluorescent polystyrene microbeads and has been applied for the simultaneous detection of three main and serious allergens (hazelnut, peanut and walnut). The method is based on the simultaneous identification of specific DNA sequences after PCR. The PCR products are biotinylated and hybridized to nut-specific oligonucleotide probes attached to the microbeads. The hybrids are finally detected by a streptavidin-phycoerythrin conjugate and flow cytometric analysis. As low as 0.01% content of the three nuts in processed food (cookies) can be detected by this method. Graphical abstract Schematic of a method for simultaneous fluorometric detection of three nut allergens in processed food (cookies) based on the use of fluorescent microbeads and flow cytometric analysis. B: Biotin; SA-PE: Streptavidin conjugated to phycoerythrin.

Keywords: DNA; Flow cytometry; Fluorescence; Microbeads; Microspheres; PCR.

PubMed Disclaimer

References

    1. Anal Bioanal Chem. 2015 May;407(14):4195-206 - PubMed
    1. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Apr;26(4):409-18 - PubMed
    1. J Agric Food Chem. 2011 May 25;59(10):5231-9 - PubMed
    1. Anal Bioanal Chem. 2010 Mar;396(5):1831-9 - PubMed
    1. Crit Rev Food Sci Nutr. 2016 Nov 17;56(15):2511-2542 - PubMed