Selective extraction of theophylline from plasma by copper-doped magnetic microspheres prior to its quantification by HPLC

Abstract

The authors describe the preparation of copper-doped magnetic microspheres (Cu-Fe₃O₄) by a solvothermal method. Due to their good magnetic property and high affinity for compounds containing an imidazole moiety (containing N-H), they are excellent adsorbents for such compounds as tested by eighteen compounds. Specifically, a method has been developed for magnetic solid-phase extraction (MSPE) of theophylline (TP) from plasma. The method enables selective enrichment of TP over many potential interferents that can occur in plasma. Following elution with alkaline methanol, TP was quantified by HPLC-UV at a detection wavelength of 272 nm. Under the optimized conditions, a linear response is found for the 0.02 to 20 μg·mL^-1 concentration range, and the limit of detection is as low as 3 ng·mL^-1. Recoveries from spiked samples range from 91.2 to 100.4%, and the repeatabilities are between 2.9 and 12% (for n = 6). The method was successfully applied to the determination of TP in rabbit and rat plasma. Graphical abstract Copper-doped magnetic microspheres are described that show good magnetic property and high affinity for compounds containing an imidazole moiety (containing an N-H group). They were successfully applied to the selective extraction of theophylline in plasma.

Keywords: Compounds containing an imidazole moiety; Cu-Fe3O4; High performance liquid chromatography; Magnetic adsorbent; Magnetic solid-phase extraction; Rabbit plasma; Rat plasma; Solvothermal method.