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Comment
. 2018 Apr;15(4):238-239.
doi: 10.1038/nmeth.4541. Epub 2018 Mar 30.

Response to "Unexpected mutations after CRISPR-Cas9 editing in vivo"

Caleb A Lareau  1   2   3 , Kendell Clement  1   3   4   5 , Jonathan Y Hsu  1   6 , Vikram Pattanayak  1   4 , J Keith Joung  1   4 , Martin J Aryee  1   2   3   4 , Luca Pinello  1   3   4
Affiliations
Comment

Response to "Unexpected mutations after CRISPR-Cas9 editing in vivo"

Caleb A Lareau et al. Nat Methods. 2018 Apr.
No abstract available

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Conflict of interest statement

Conflict of interest statement

J.K.J. has financial interests in Beam Therapeutics, Editas Medicine, Monitor Biotechnologies, Pairwise Plants, Poseida Therapeutics, and Transposagen Biopharmaceuticals. M.J.A. has financial interests in Monitor Biotechnologies. J.K.J.’s and M.J.A.’s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies.

Figures

Figure 1.
Figure 1.. Measures of genetic relatedness in the F03, F05 and FVB mice.
(a) Isogenic model of 3 mice with no private mutations within or shared between mice. (b) observed dbSNP in F03 and F05 mice (n = 31,079). (c) an isogenic system assumes the number of loci with shared genotypes is nearly identical for all mice (d) the observed data demonstrates a clear departure from this equal genetic model at common variants and other non-dbSNP loci (n = 38,981). The variants previously reported by Schaefer et al. (dark gray) represent only a small subset of the genotypes common to F03 and F05 but distinct from FVB at non-dbSNP sites. The observed ratios in B and D cannot be distinguished from each other (p = 0.304; two-sided Fisher’s Exact Test), but each represent a significant departure (p < 2.2 × 10−16; Chi-Squared Test) from the equal genetic distance model (C) required to attribute differential SNVs to Cas9 activity.
See this image and copyright information in PMC

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References

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    1. Iyer V et al. Off-target mutations are rare in Cas9-modified mice. Nat Methods 12, 479 (2015). - PubMed

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