doi: 10.1016/0003-2697(87)90501-x.
Method for exchange of the lipid environment of the membrane-bound (Mg2+ + Na+)-ATPase of Acholeplasma laidlawii B
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- PMID: 2960250
- DOI: 10.1016/0003-2697(87)90501-x
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Method for exchange of the lipid environment of the membrane-bound (Mg2+ + Na+)-ATPase of Acholeplasma laidlawii B
Anal Biochem.
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Abstract
A method that exchanges the endogenous lipids in the environment of the membrane-bound (Mg2+ + Na+)-ATPase of Acholeplasma laidlawii B with defined exogenous lipids has been devised. Results demonstrate that 99.9% of the original membrane lipids were replaced with phosphatidylcholine and phosphatidic acid. ATPase enzyme activity was maintained throughout the substitution procedure.
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