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. 2018 Dec;43(12):1134.e1-1134.e6.
doi: 10.1016/j.jhsa.2018.02.033. Epub 2018 Mar 28.

Tendon Adhesions: A Novel Method of Objectively Measuring Adhesions by Assessing Tendon Glide Through a Soft Tissue Envelope in a Rat Model

Aimee Riley  1 , Jonathan E Isaacs  2 , Ilvy Cotterell  1 , Jeffrey Stromberg  1 , Satya Mallu  1 , Gaurangkumar Patel  1
Affiliations

Tendon Adhesions: A Novel Method of Objectively Measuring Adhesions by Assessing Tendon Glide Through a Soft Tissue Envelope in a Rat Model

Aimee Riley et al. J Hand Surg Am. 2018 Dec.

Abstract

Purpose: To develop a rat model of extra-synovial tendon adhesions that will enable accurate testing of scar barriers and adhesion inhibiting treatments to facilitate future research.

Methods: Thirty-six 6-month-old male Sprague-Dawley rats were randomized to one of the 3 groups of 12. In Group A, the middle one-third portion of the left Achilles tendon was excised. In Group B, the tendon and soft tissue bed was abraded with steel wool. In Group C, a silk suture was sewn along the tendon. The right hind limbs served as controls. At 4 weeks, biomechanical testing was performed on the bilateral hind limbs. The Achilles tendon was cut at the gastrocnemius-tendon junction proximal to the "adhesion zone" (or analogous level in the control limb). The calcaneal insertion of the Achilles was attached to a tensiometer. The force needed to pull the tendon out of its soft tissue envelope at a fixed rate was measured.

Results: Three rats were excluded because of complications during data collection. Pair-wise comparison testing was performed, comparing the mean peak force to pull the Achilles tendon from its soft tissue envelope in 33 control limbs and the contralateral limb from each group. The average peak force for the cut tendon group (A) was 20.1 N, 18.8 N in the steel wool group (B), and 21.1 N in the suture group (C). The average peak force in the control limbs was 15.6 N. There was a significant difference noted in peak forces between the control limbs and each experimental group.

Conclusions: A consistent and statistically increased force was necessary to pull a rodent Achilles tendon from an adhesion-induced tissue bed compared with controls. No statistical difference was detected between experimental groups.

Clinical relevance: Our study demonstrates an objective method of biomechanical tendon adhesion assessment in a rat model.

Keywords: Adhesion; biomechanical; rodent; tendon.

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