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. 2018 Sep;179(3):792-794.
doi: 10.1111/bjd.16600. Epub 2018 Jun 29.

Collagen deposition in chronic hidradenitis suppurativa: potential role for CD163+ macrophages

A S Byrd  1 , M L Kerns  1 , D W Williams  2   3 , J C Zarif  4   5 , A Z Rosenberg  6 , M Delsante  6 , H Liu  1 , C A Dillen  1 , J P Maynard  6 , J A Caffrey  7 , J M Sacks  7 , S M Milner  7 , O Aliu  7 , K P Broderick  7 , L S Lew  1 , L S Miller  1 , S Kang  1 , G A Okoye  1
Affiliations

Collagen deposition in chronic hidradenitis suppurativa: potential role for CD163+ macrophages

A S Byrd et al. Br J Dermatol. 2018 Sep.
No abstract available

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Conflict of interest statement

Conflict of Interest: Dr. Ginette A. Okoye is a consultant for AbbVie.

Figures

Figure 1
Figure 1. Immunohistochemistry, flow cytometry, and immunofluorescence findings of chronic HS lesions
(a) Perilesional and chronic lesional HS tissue sections demonstrate dermal infiltration of inflammatory cells, including macrophages in affected areas (n=15, H&E). (b) Immunohistochemistry demonstrates a CD163+ infiltrate in the dermis of in HS chronic lesional tissue (n=8, 20x, scale bar 100 μm outer panels; 10x, scale bar 250 μm middle panel), especially surrounding hair follicles (black arrowheads, 20x, scale bar 100 μm). (c) Quantification of CD163 protein expression in perilesional and lesional regions (mean ± SD, *p=0.0357, Mann-Whitney U test). (d) Flow cytometry demonstrates a 29.4% increase in CD68+C163+ cells in chronic HS lesions (n=3, pink) compared to normal control tissue (n=2, grey). Live single cells were used to set population gates followed by selecting CD68+ cells. (e) Indirect immunofluorescence for CCL18 in chronic HS lesions (n=5), normal control tissue (n=3) and perilesional tissue (n=3) (10x, scale bar 250 μm). (f) Double indirect immunofluorescence staining for CD163 (red), CCL18 (green), and DAPI for nuclei staining (blue). The images for CD163 and CCL18 were merged (yellow-gold) (n=5, 20x, scale bar 100 μm). (g) HS chronic lesional tissue (n=5) show inflammatory infiltrate (black arrow) adjacent to collagen (yellow asterisks, blue stained material) as well as near a hair follicle (black arrowhead) (10x, scale bar 250 μm, perilesional (n=5), Masson’s trichrome (MT)). (h) Picrosirius Red (PSR) staining shows more organized thick, mature, dense bundles of collagen in perilesional tissue (n=1) and thinner, less mature, loose collagen bundles in the chronic lesional HS tissue (n=2) (white arrows, 20x, scale bar 250 μm). (i) Mixed infiltrate including macrophages (black arrow) and reactive myofibroblasts (black double arrow) are present in chronic lesional tissue, 60x. (j) Schematic of proposed role for macrophages in chronic HS: CD68+CD163+ macrophage release of CCL18 stimulates fibroblast-mediated collagen deposition as seen clinically in chronic HS lesions.
See this image and copyright information in PMC

References

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