doi: 10.1128/jb.169.12.5880-5883.1987.
Mutational analysis of the lambda int gene: DNA sequence of dominant mutations
Affiliations
- PMID: 2960665
- PMCID: PMC214194
- DOI: 10.1128/jb.169.12.5880-5883.1987
Item in Clipboard
Mutational analysis of the lambda int gene: DNA sequence of dominant mutations
J Bacteriol.
1987 Dec.
Abstract
We have combined techniques of genetic and physical mapping with rapid DNA sequence analysis to identify the nucleotide change in lambda int mutations. These mutations define two dominant phenotypic classes: (i) recombination that is partially independent of accessory factors, and (ii) inhibition of wild-type Int by missense or nonsense proteins, i.e., negative complementation.
Similar articles
-
Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.Annu Rev Genet. 1981;15:143-67. doi: 10.1146/annurev.ge.15.120181.001043. Annu Rev Genet. 1981. PMID: 6461289 Review. No abstract available.
-
Site-specific recombination functions of bacteriophage lambda: DNA sequence of regulatory regions and overlapping structural genes for Int and Xis.Proc Natl Acad Sci U S A. 1980 May;77(5):2482-6. doi: 10.1073/pnas.77.5.2482. Proc Natl Acad Sci U S A. 1980. PMID: 6446713 Free PMC article.
-
Features of bacteriophage lambda: analysis of the complete nucleotide sequence.Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1009-24. doi: 10.1101/sqb.1983.047.01.115. Cold Spring Harb Symp Quant Biol. 1983. PMID: 6222866 No abstract available.
-
Lambda site-specific recombination: the att site.Cell. 1981 Sep;25(3):585-6. doi: 10.1016/0092-8674(81)90165-3. Cell. 1981. PMID: 6456816 No abstract available.
-
[Bacteriophage lambda integration into host chromosome (biochemistry of Int protein and pleiotropic effects of host factors) (author's transl)].Tanpakushitsu Kakusan Koso. 1980;25(1):49-64. Tanpakushitsu Kakusan Koso. 1980. PMID: 6243784 Review. Japanese. No abstract available.
Cited by
-
Two structural features of lambda integrase that are critical for DNA cleavage by multimers but not by monomers.Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2770-5. doi: 10.1073/pnas.0400135101. Epub 2004 Feb 19. Proc Natl Acad Sci U S A. 2004. PMID: 14976241 Free PMC article.
-
Integration host factor of Escherichia coli reverses the inhibition of R6K plasmid replication by pi initiator protein.J Bacteriol. 1991 Feb;173(3):1279-86. doi: 10.1128/jb.173.3.1279-1286.1991. J Bacteriol. 1991. PMID: 1991721 Free PMC article.
-
Nucleotide sequence and genetic characterization of staphylococcal bacteriophage L54a int and xis genes.J Bacteriol. 1989 Aug;171(8):4146-53. doi: 10.1128/jb.171.8.4146-4153.1989. J Bacteriol. 1989. PMID: 2526804 Free PMC article.
-
A point mutation in the Nul gene of bacteriophage lambda facilitates phage growth in Escherichia coli with himA and gyrB mutations.Mol Gen Genet. 1988 Apr;212(1):149-56. doi: 10.1007/BF00322458. Mol Gen Genet. 1988. PMID: 2836702
-
An intermediate in the phage lambda site-specific recombination reaction is revealed by phosphorothioate substitution in DNA.Nucleic Acids Res. 1988 Jul 25;16(14B):6839-56. doi: 10.1093/nar/16.14.6839. Nucleic Acids Res. 1988. PMID: 2970060 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
