In-vitro biosynthesis of C18 neutral steroids in horse testes
Affiliations
- PMID: 2960805
Item in Clipboard
In-vitro biosynthesis of C18 neutral steroids in horse testes
J Reprod Fertil Suppl.
1987.
Abstract
Deuterium, 14C- and 3H-labelled steroid substrates were incubated with minced testicular tissue from stallions of different ages. After extraction and separation of the neutral and phenolic fractions the metabolites were identified by gas chromatography-mass spectrometry. The presence of the expected C19 neutral and C18 phenolic steroids was confirmed. An isomer of 5(10)-oestrene-3,17-diol was also identified.
Similar articles
-
Steroids in equine testes: the identification of endogenous 19-hydroxy and 19-nor neutral steroids by gas chromatography--mass spectrometry.J Endocrinol. 1989 Feb;120(2):223-9. doi: 10.1677/joe.0.1200223. J Endocrinol. 1989. PMID: 2926297
-
The use of stable isotopes and gas chromatography/mass spectrometry in the identification of steroid metabolites in the equine.Steroids. 1990 Oct;55(10):433-9. doi: 10.1016/0039-128x(90)90011-y. Steroids. 1990. PMID: 2149219
-
Studies related to the origin of C18 neutral steroids isolated from extracts of urine from the male horse: the identification of urinary 19-oic acids and their decarboxylation to produce estr-4-en-17beta-ol-3-one (19-nortestosterone) and estr-4-ene-3,17-dione (19-norandrost-4-ene-3,17-dione) during sample processing.Anal Chim Acta. 2007 Mar 14;586(1-2):196-207. doi: 10.1016/j.aca.2006.11.059. Epub 2006 Dec 1. Anal Chim Acta. 2007. PMID: 17386712
-
Studies related to the metabolism of anabolic steroids in the horse: a gas chromatographic mass spectrometric method to confirm the administration of 19-nortestosterone or its esters to horses.Biomed Mass Spectrom. 1978 Feb;5(2):170-3. doi: 10.1002/bms.1200050213. Biomed Mass Spectrom. 1978. PMID: 564720
-
[Protein binding of steroids].Rev Fr Gynecol Obstet. 1984 Feb;79(2):79-84, 87-9. Rev Fr Gynecol Obstet. 1984. PMID: 6398900 Review. French.