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. 2018 Mar 26:24:252-260.
eCollection 2018.

Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment

Nannan Ding  1 , Shasha Luo  1 , Jinjin Yu  1 , Ying Zhou  1 , Zhifeng Wu  1
Affiliations

Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment

Nannan Ding et al. Mol Vis. .

Abstract

Purpose: This study aims to quantify the concentration of apolipoprotein A1 (APOA1) and retinol binding protein (RBP4) expressed in the vitreous humors of patients with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD), rhegmatogenous retinal detachment (RRD), and idiopathic epimacular membrane (IEM). This study also aims to investigate the potential role of APOA1 and RBP4 as biomarkers of RRDCD.

Methods: Enzyme-linked immunosorbent assay (ELISA) kits were used to obtain levels of APOA1 and RBP4 from the vitreous humor samples of 76 primary patients. These patients included 23 patients with RRDCD, 28 patients with RRD, and 24 patients with IEM. All patients were undergoing planned pars plana vitrectomy. The differences between the concentrations of the molecular biomarkers among different patient groups were analyzed using the Mann-Whitney U-test for nonparametric values and independent samples t-test or one-way ANOVA analysis for parametric data. The relationship between the molecular biomarkers, grades of proliferative vitreoretinopathy (PVR), and quadrants of retinal detachment were analyzed using nonparametric Spearman's rank correlation analysis.

Results: The vitreous concentrations of APOA1 and RBP4 were statistically significantly higher in the RRDCD group compared to the RRD and IEM groups. Patients with severe PVR demonstrated a higher concentration of APOA1 and RBP4 compared to those with mild PVR, but this finding was not statistically significant. There was a statistically significant positive correlation between APOA1 and RBP4 in the RRDCD and RRD groups. Nonparametric Spearman's rank correlation analysis revealed that levels of APOA1 and RBP4 increased statistically significantly with an increasing number of detached retinal quadrants in the RRDCD and RRD groups.

Conclusions: The findings of this study allude to the potential of APOA1 and RBP4 as specific biomarkers of RRDCD. The findings of this study may contribute to increased understanding regarding the role of APOA1 and RBP4 in RRDCD.

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Figures

Figure 1
Figure 1
Scatter plots of concentration of APOA1 and RBP4. Scatter plots of concentrations of APOA1 (A) and RBP4 (B). The difference in the APOA1 and RBP4 concentrations between groups was statistically significant (p<0.001). Pairwise comparison among groups also showed that the difference in the APOA1 and RBP4 concentrations was statistically significant (p<0.05).
Figure 2
Figure 2
The relationship between the concentrations of APOA1 (R=0.426, p=0.043) and RBP4 (R=0.574, p=0.004) in the vitreous and the extent of retinal detachment in patients with RRDCD.
Figure 3
Figure 3
The relationship between the concentrations of APOA1 (R=0.426, p=0.024) and RBP4 (R=0.397, p=0.036) in the vitreous and the extent of retinal detachment in patients with RRD.
Figure 4
Figure 4
Correlation between APOA1 and RBP4 in each group. APOA1/RBP4 (R=0.594, p=0.003) in the RRDCD group (A) and APOA1/RBP4 (R=0.708, p<0.001) in the RRD group (B). These results confirm the presence of statistically significant positive correlations between APOA1 and RBP4 in the RRDCD and RRD groups.
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References

    1. Jarrett WH., 2nd Rhematogenous retinal detachment complicated by severe intraocular inflammation, hypotony, and choroidal detachment. Trans Am Ophthalmol Soc. 1981;79:664–83. - PMC - PubMed
    1. Wei Y, Wang N, Chen F, Wang H, Bi C, Zu Z, Yang X. Vitrectomy combined with periocular/intravitreal injection of steroids for rhegmatogenous retinal detachment associated with choroidal detachment. Retina. 2014;34:136–41. - PubMed
    1. Sharma T, Gopal L, Reddy RK, Kasinathan N, Shah NA, Sulochana KN, Miriam KC, Arvind K, Ramakrishnan S, Sukumar B. Primary vitrectomy for combined rhegmatogenous retinal detachment and choroidal detachment with or without oral corticosteroids: a pilot study. Retina. 2005;25:152–7. - PubMed
    1. Zhu J, Xu X, Zhang X. Surgical therapeutic results of rhegmatogenous retinal detachment associated with choroidal detachment. Zhonghua Yan Ke Za Zhi. 2002;38:135–9. - PubMed
    1. Li Z, Li Y, Huang X, Cai XY, Chen X, Li S, Huang Y, Lu L. Quantitative analysis of rhegmatogenous retinal detachment associated with choroidal detachment in Chinese using UBM. Retina. 2012;32:2020–5. - PubMed

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