doi: 10.1007/BF00230146.
A novel stimulator of protein phosphorylation in Neurospora crassa
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- PMID: 2961977
- DOI: 10.1007/BF00230146
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A novel stimulator of protein phosphorylation in Neurospora crassa
Mol Cell Biochem.
1987 Sep.
Abstract
An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100,000 X g supernatants of Neurospora crassa mycelial extracts. This 38,000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed.
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