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. 2018 Mar;13(3):410-412.
doi: 10.4103/1673-5374.228716.

Linking axon transport to regeneration using in vitro laser axotomy

Bart Nieuwenhuis  1 , Richard Eva  2
Affiliations

Linking axon transport to regeneration using in vitro laser axotomy

Bart Nieuwenhuis et al. Neural Regen Res. 2018 Mar.
No abstract available

PubMed Disclaimer

Conflict of interest statement

None declared

Figures

Figure 1
Figure 1
Cell culture model to study the intrinsic axonal regeneration response. (A) A three-step set-up to investigate axonal regeneration in cultured neurons. In this example, primary cortical neurons were plated on a glass-bottomed imaging dish and were transfected with various DNA plasmids. The axons of the transfected neurons underwent laser axotomy and were video recorded for 14 hours. The arrow shows the site of injury. (B) Representative images of a non-regenerating axon (upper panels) and a regenerating axon (lower panels) from cortical neurons that were cultured for two weeks. The asterisk indicates the location of the proximal stump after one-hour post-axotomy, while the arrow marks the growth cone at indicated time-point. There is no difference in distal degeneration rate when comparing between regenerating and non-regenerating axons. Scale bars: 50 μm.
Figure 2
Figure 2
Enhancing axonal regeneration of central nervous system neurons by targeting selective transport. (A) Endogenous distribution of trafficking-associated molecules and integrins in 14 days in vitro (DIV) primary cortical neurons. (B) Summary of the trafficking intervention by Koseki et al. (2017) and Eva et al. (2017) that promotes axonal regeneration in central nervous system neurons in vitro.
See this image and copyright information in PMC

References

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