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. 2018 May 16;29(5):1500-1504.
doi: 10.1021/acs.bioconjchem.8b00173. Epub 2018 Apr 12.

Creation of Linear Carbon Dot Array with Improved Optical Properties through Controlled Covalent Conjugation with DNA

Sonam Kumari  1 , Apurv Solanki  2 , Saptarshi Mandal  1 , Deepa Subramanyam  2 , Prolay Das  1
Affiliations

Creation of Linear Carbon Dot Array with Improved Optical Properties through Controlled Covalent Conjugation with DNA

Sonam Kumari et al. Bioconjug Chem. .

Abstract

Controlled conjugation of fluorescent carbon dots (CDs) with DNA and subsequent fabrication of the CDs into an array through hybridization mediated self-assembly in the solution phase is reported. Covalent conjugation of CD with DNA and the subsequent array formation change the mobility of the CD-DNA array in gel electrophoresis and HPLC significantly. Interspatial distance in the CD-DNA array is tuned by the DNA sequence length and maintained at ∼8 ± 0.3 nm as revealed by electron microscopy studies. An increase in fluorescence lifetime by ∼2 ns was observed for the CD-DNA array compared to a solitary CD, vis-á-vis better imaging prospects of HEK293 cells by the former. Thus, the array displays improved fluorescence and unhindered cell penetration.

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Conflict of interest statement

Notes

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
A. Agarose gel image showing the mobility of conjugation products of DNA (S1) with CD in various ratios. B. Agarose gel image showing mobility of purified CD array after hybridization.
Figure 2
Figure 2
A. UV–vis absorption spectra. B. Steady-state fluorescence spectra. C. Time-resolved fluorescence spectra of CD, (S1)2-CD, and [(S1)2-CD-(S2)2-CD]n array. D. Circular dichroism spectra.
Figure 3
Figure 3
A and B. AFM image of CD and [(S1)2-CD-(S2)2-CD]n array. C and D. TEM images of CD and [(S1)2-CD-(S2)2-CD]n array.
Figure 4
Figure 4
Confocal images of HEK293 cells incubated with CD and CD-Array.
Scheme 1
Scheme 1. Scheme for Creation of DNA Templated CD Array through Hybridization
See this image and copyright information in PMC

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