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. 1988 Jan;211(1):95-101.
doi: 10.1007/BF00338398.

Mapping and regulation of the cel genes in Erwinia chrysanthemi

J L Aymeric  1 , A Guiseppi, M C Pascal, M Chippaux
Affiliations

Mapping and regulation of the cel genes in Erwinia chrysanthemi

J L Aymeric et al. Mol Gen Genet. 1988 Jan.

Abstract

Chromosomal mutations of the celZ and celY genes which encode two different endoglucanases in Erwinia chrysanthemi 3937 were obtained by a three-step procedure: (i) in Escherichia coli, insertions of lacZ fusion-forming mini-Mu bacteriophages in the cel genes cloned on plasmids and screening of cel-lac fusions, (ii) Mu-mediated transduction in E. chrysanthemi of the plasmids carrying the fusions, (iii) recombinational exchange between the plasmidic mutated and the wild-type chromosomal alleles. These mutations allowed mapping of celZ between ura and pan and celY between xyl and met on the linkage map of E. chrysanthemi. The beta-galactosidase activity of these strains indicated that celZ is expressed in the late exponential and stationary growth phases, while celY expression is almost undetectable.

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References

    1. J Bacteriol. 1985 Oct;164(1):390-6 - PubMed
    1. J Bacteriol. 1985 Oct;164(1):51-6 - PubMed
    1. J Bacteriol. 1982 Apr;150(1):122-31 - PubMed
    1. Gene. 1979 May;6(1):23-8 - PubMed
    1. Anal Biochem. 1984 Jun;139(2):383-9 - PubMed

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