In vitro immunization for antibody production against tetanus toxin and toxoid. 3. Recombinant gamma interferon and indomethacin support specific antibody production induced by antigen-pulsed immobilized monocytes

Abstract

Specific antibody production against Tetanus Toxin by in vitro cultured human peripheral blood lymphocytes may be enhanced, when monocytes were immobilized onto FCS-coated culture plates and then pulsed with Tetanus Toxoid. Using this model several immunomodulators were tested for their influence on in vitro immunization efficiency. A combination of recombinant gamma interferon and indomethacin added during antigen presentation to lymphocytes, was able to potentiate specific antibody response.