Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA
- PMID: 2964277
- DOI: 10.1016/s0092-8674(88)80034-5
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA
Abstract
A novel strategy has been used to isolate a cDNA clone that encodes a DNA binding domain whose recognition properties overlap those of the mammalian transcription factors H2TF1 and NF-kappa B. These two factors are distinguished by their cell type distributions and their relative affinities for related sequence elements in the enhancers of the major histocompatibility complex (MHC) class I and immunoglobulin kappa chain genes. The human cDNA clone was detected by screening a lambda phage expression library with a binding site probe derived from the MHC enhancer. The phage encoded fusion protein binds specifically to both the MHC and kappa gene enhancers. The cDNA hybridizes to a single copy gene that is expressed as a 10 kb mRNA in both B and non-B cells. The strategy used in this study may prove generally useful in the cloning and analysis of sequence-specific DNA binding proteins.
Similar articles
-
Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter.Proc Natl Acad Sci U S A. 1988 Feb;85(3):723-7. doi: 10.1073/pnas.85.3.723. Proc Natl Acad Sci U S A. 1988. PMID: 3422454 Free PMC article.
-
A large protein containing zinc finger domains binds to related sequence elements in the enhancers of the class I major histocompatibility complex and kappa immunoglobulin genes.Mol Cell Biol. 1990 Apr;10(4):1406-14. doi: 10.1128/mcb.10.4.1406-1414.1990. Mol Cell Biol. 1990. PMID: 2108316 Free PMC article.
-
Two genes encode factors with NF-kappa B- and H2TF1-like DNA-binding properties.Proc Natl Acad Sci U S A. 1990 Nov;87(22):8707-10. doi: 10.1073/pnas.87.22.8707. Proc Natl Acad Sci U S A. 1990. PMID: 2247438 Free PMC article.
-
Altered binding of regulatory factors to HLA class I enhancer sequence in human tumor cell lines lacking class I antigen expression.Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3488-92. doi: 10.1073/pnas.89.8.3488. Proc Natl Acad Sci U S A. 1992. PMID: 1565643 Free PMC article.
-
The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.Cell. 1990 Sep 7;62(5):1007-18. doi: 10.1016/0092-8674(90)90275-j. Cell. 1990. PMID: 2203531
Cited by
-
A tobacco bZip transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes.EMBO J. 1991 Jul;10(7):1793-802. doi: 10.1002/j.1460-2075.1991.tb07704.x. EMBO J. 1991. PMID: 2050116 Free PMC article.
-
Purification and properties of the Rous sarcoma virus internal enhancer binding factor.Mol Cell Biol. 1989 May;9(5):1929-39. doi: 10.1128/mcb.9.5.1929-1939.1989. Mol Cell Biol. 1989. PMID: 2546054 Free PMC article.
-
An inducible 50-kilodalton NF kappa B-like protein and a constitutive protein both bind the acute-phase response element of the angiotensinogen gene.Mol Cell Biol. 1990 Mar;10(3):1023-32. doi: 10.1128/mcb.10.3.1023-1032.1990. Mol Cell Biol. 1990. PMID: 2106065 Free PMC article.
-
Cyclic AMP-dependent protein kinase regulates transcription of the phosphoenolpyruvate carboxykinase gene but not binding of nuclear factors to the cyclic AMP regulatory element.Mol Cell Biol. 1990 Jul;10(7):3357-64. doi: 10.1128/mcb.10.7.3357-3364.1990. Mol Cell Biol. 1990. PMID: 2141384 Free PMC article.
-
Multiple cDNA clones encoding nuclear proteins that bind to the tax-dependent enhancer of HTLV-1: all contain a leucine zipper structure and basic amino acid domain.EMBO J. 1990 Aug;9(8):2537-42. doi: 10.1002/j.1460-2075.1990.tb07434.x. EMBO J. 1990. PMID: 2196176 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
