Molecular cloning and sequencing of sucrose synthase cDNA from potato (Solanum tuberosum L.): preliminary characterization of sucrose synthase mRNA distribution

Abstract

A cDNA library constructed from poly(A)+ RNA from potato tuber in lambda gt11 was screened for sucrose synthase using a maize sucrose synthase cDNA probe. The longest 2.7-kb insert was sequenced. An ATG located within the sequence CTGCAATGG starts an open reading frame of 805 codons. The nucleotide sequence, when compared to the maize sucrose synthase cDNA sequence, exhibits about 70% identity and the deduced amino acid sequence about 75%. Three amino acid regions are about 90% homologous and two of them could be important for the protein function. Expression studies show that transcription of the potato sucrose synthase gene is at least ten fold higher in tubers compared to photosynthetically active tissues.