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. 2018 Apr 12;13(4):e0194259.
doi: 10.1371/journal.pone.0194259. eCollection 2018.

Serum microRNA profiles in athyroid patients on and off levothyroxine therapy

Elske T Massolt  1   2 , Layal Chaker  1   2   3 , Theo J Visser  1   2 , Ad J M Gillis  4 , Lambert C J Dorssers  4 , Carolien M Beukhof  1   2 , Boen L R Kam  5 , Gaston J Franssen  6 , Giulia Brigante  1   2   7 , Tessa M van Ginhoven  6 , W Edward Visser  1   2 , Leendert H J Looijenga  4 , Robin P Peeters  1   2
Affiliations

Serum microRNA profiles in athyroid patients on and off levothyroxine therapy

Elske T Massolt et al. PLoS One. .

Abstract

Background: Levothyroxine replacement treatment in hypothyroidism is unable to restore physiological thyroxine and triiodothyronine concentrations in serum and tissues completely. Normal serum thyroid stimulating hormone (TSH) concentrations reflect only pituitary euthyroidism and, therefore, novel biomarkers representing tissue-specific thyroid state are needed. MicroRNAs (miRNAs), small non-coding regulatory RNAs, exhibit tissue-specific expression patterns and can be detectable in serum. Previous studies have demonstrated differential expression of (precursors of) miRNAs in tissues under the influence of thyroid hormone.

Objective: To study if serum miRNA profiles are changed in different thyroid states.

Design and methods: We studied 13 athyroid patients (6 males) during TSH suppressive therapy and after 4 weeks of thyroid hormone withdrawal. A magnetic bead capture system was used to isolate 384 defined miRNAs from serum. Subsequently, the TaqMan Array Card 3.0 platform was used for profiling after individual target amplification.

Results: Mean age of the subjects was 44.0 years (range 20-61 years). Median TSH levels were 88.9 mU/l during levothyroxine withdrawal and 0.006 mU/l during LT4 treatment with a median dosage of 2.1 μg/kg. After normalization to allow inter-sample analysis, a paired analysis did not demonstrate a significant difference in expression of any of the 384 miRNAs analyzed on and off LT4 treatment.

Conclusion: Although we previously showed an up-regulation of pri-miRNAs 133b and 206 in hypothyroid state in skeletal muscle, the present study does not supply evidence that thyroid state also affects serum miRNAs in humans.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Heat map showing the Cq values of all detectable MiRNAs (n = 135) in the different thyroid states.
Clustering did not group the samples according to thyroid state.
See this image and copyright information in PMC

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