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. 2018 Apr 12;15(1):69.
doi: 10.1186/s12985-018-0980-0.

Enterovirus serotypes in patients with central nervous system and respiratory infections in Viet Nam 1997-2010

Nguyen Thi Thuy Chinh B'Krong  1   2 , Ngo Ngoc Quang Minh  1   3 , Phan Tu Qui  1   3   4 , Tran Thi Hong Chau  1   4 , Ho Dang Trung Nghia  1   4 , Lien Anh Ha Do  1   5 , Nguyen Ngoc Nhung  1   6 , Nguyen Van Vinh Chau  4 , Guy Thwaites  1   7 , Le Van Tan  1 , H Rogier van Doorn  8   9   10 , Tran Tan Thanh  11
Affiliations

Enterovirus serotypes in patients with central nervous system and respiratory infections in Viet Nam 1997-2010

Nguyen Thi Thuy Chinh B'Krong et al. Virol J. .

Abstract

Background: Enteroviruses are the most common causative agents of human illness. Enteroviruses have been associated with regional and global epidemics, recently, including with severe disease (Enterovirus A71 and D68), and are of interest as emerging viruses. Here, we typed Enterovirus A-D (EV) from central nervous system (CNS) and respiratory infections in Viet Nam.

Methods: Data and specimens from prospective observational clinical studies conducted between 1997 and 2010 were used. Species and serotypes were determined using type-specific RT-PCR and viral protein 1 or 4 (VP1, VP4) sequencing.

Results: Samples from patients with CNS infection (51 children - 10 CSF and 41 respiratory/rectal swabs) and 28 adults (28 CSF) and respiratory infection (124 children - 124 respiratory swabs) were analysed. Twenty-six different serotypes of the four Enterovirus species (A-D) were identified, including EV-A71 and EV-D68. Enterovirus B was associated with viral meningitis in children and adults. Hand, foot and mouth disease associated Enteroviruses A (EV-A71 and Coxsackievirus [CV] A10) were detected in children with encephalitis. Diverse serotypes of all four Enterovirus species were found in respiratory samples, including 2 polio-vaccine viruses, but also 8 CV-A24 and 8 EV-D68. With the exception of EV-D68, the relevance of these viruses in respiratory infection remains unknown.

Conclusion: We describe the diverse spectrum of enteroviruses from patients with CNS and respiratory infections in Viet Nam between 1997 and 2010. These data confirm the global circulation of Enterovirus genera and their associations and are important for clinical diagnostics, patient management, and outbreak response.

Keywords: Central nervous system infection; Enterovirus; Respiratory infection; Serotyping.

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Conflict of interest statement

Ethics approval and consent to participate

The original studies were reviewed and approved by the local Institutional Review Boards of all enrolling hospitals and the Oxford Tropical Research Ethics Committee (OxTREC), University of Oxford, Oxford, United Kingdom. Written informed consent was obtained from parent or legal guardian of each participant. Subsequent approvals for use of original samples for serotyping of enterovirus was obtained from the Institutional Review Boards from the enrolling hospitals when not part of the original protocols.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Monthly distribution of cases with EV detection
See this image and copyright information in PMC

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