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. 2018 Apr;33(2):218-221.
doi: 10.1007/s12291-017-0658-1. Epub 2017 May 2.

Plasma Neutrophil Elastase, α1-Antitrypsin, α2-Macroglobulin and Neutrophil Elastase-α1-Antitrypsin Complex Levels in patients with Dengue Fever

Mamatha Kunder  1 , V Lakshmaiah  2 , A V Moideen Kutty  1
Affiliations

Plasma Neutrophil Elastase, α1-Antitrypsin, α2-Macroglobulin and Neutrophil Elastase-α1-Antitrypsin Complex Levels in patients with Dengue Fever

Mamatha Kunder et al. Indian J Clin Biochem. 2018 Apr.

Abstract

Dengue fever (DF) is characterized by systemic inflammatory response including neutrophil activation leading to uncontrolled elastase activity. This study was aimed to measure the activity of plasma neutrophil elastase (NE), its endogenous inhibitors α1-antitrypsin (α1-AT) and α2-macroglobulin (α2-MG) and elastase in complex with α1-AT (NE-α1-AT complex) in DF. 50 dengue patients [39 DF and 11 dengue hemorrhagic fever (DHF)] and 52 healthy subjects were included in the study. NE was measured using N-succinyl-tri-alanine-p-nitroanilide as substrate. α1-AT, α2-MG and NE-α1-AT complex were estimated by ELISA. The result analysis indicated that the dengue patients had significantly higher elastase activity with significantly reduced inhibitor levels compared to controls. Between DF and DHF patients, DHF group had significantly higher elastase activity. In conclusion, significantly elevated NE and reduced inhibitors level in dengue fever indicate these parameters could be of significance in DF particularly for the assessment of progression of inflammatory processes.

Keywords: Dengue fever; NE–α1-AT complex; Neutrophil elastase; α1-Antitrypsin; α2-Macroglobulin.

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Conflict of interest statement

Compliance with Ethical StandardsThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Elastase activity in DF and DHF patients
See this image and copyright information in PMC

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