Renoprotective effect of cinnamaldehyde in food color induced toxicity

Abstract

Present study reports the effects of metanil yellow, a non-permitted food colouring dye, on the biomarkers of oxidative stress and kidney function in blood and renal tissue of albino Wistar rats and its mitigation by cinnamaldehyde, a major phytoconstituents of cinnamon. Oral administration of metanil yellow in rats caused about 70% reduction in ferric reducing ability (FRAP 5.1 μM/L) and 50% decline in reduced glutathione (GSH 59.27 nM/mg protein) content in plasma with simultaneous increase in serum creatinine level. In kidney tissues, activities of superoxide dismutase (SOD), catalase and GSH dropped while malondialdehyde (MDA) content increased. Co-administration of cinnamaldehyde with metanil yellow showed considerable restorative effect on the biomarkers of plasma antioxidant status and kidney function i.e., FRAP (11.5 μM/L), GSH (83-88.5 nM/mg protein), urea, creatinine, SOD, catalase and MDA. Administration of cinnamaldehyde restored the kidney enzyme activities up to 75% of the base level. The study revealed that reno-protective action of cinnamaldehyde was mediated by lowering oxidative stress level.

Keywords: Cinnamaldehyde; Food colour; Metanil yellow; Oxidative stress; Renoprotective.

Fig. 1

Effect of cinnamaldehyde on ferric reducing ability of plasma (FRAP) of metanil yellow fed albino Wistar rats. The data represent mean ± SD (n = 6; p < 0.001). Group details: Group 1—normal control; Group 2—metanil yellow (Myl) treated; Group 3—Liv52 + Myl; Group 4—cinnamaldehyde + Myl; Group 5—vehicle control (olive oil treated)

Fig. 2

Effect of cinnamaldehyde on plasma GSH content of metanil yellow fed albino Wistar rats. The data represent mean ± SD (n = 6; p < 0.001). Group details: Group 1—normal control; Group 2—metanil yellow (Myl) treated; Group 3—Liv52 + Myl; Group 4—cinnamaldehyde + Myl; Group 5—vehicle control (olive oil treated)