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. 2018 Oct;111(10):1883-1912.
doi: 10.1007/s10482-018-1081-1. Epub 2018 Apr 13.

New Penicillium and Talaromyces species from honey, pollen and nests of stingless bees

Renan N Barbosa  1   2 , Jadson D P Bezerra  2 , Cristina M Souza-Motta  2 , Jens C Frisvad  3 , Robert A Samson  1 , Neiva T Oliveira  2 , Jos Houbraken  4
Affiliations

New Penicillium and Talaromyces species from honey, pollen and nests of stingless bees

Renan N Barbosa et al. Antonie Van Leeuwenhoek. 2018 Oct.

Abstract

Penicillium and Talaromyces species have a worldwide distribution and are isolated from various materials and hosts, including insects and their substrates. The aim of this study was to characterize the Penicillium and Talaromyces species obtained during a survey of honey, pollen and the inside of nests of Melipona scutellaris. A total of 100 isolates were obtained during the survey and 82% of those strains belonged to Penicillium and 18% to Talaromyces. Identification of these isolates was performed based on phenotypic characters and β-tubulin and ITS sequencing. Twenty-one species were identified in Penicillium and six in Talaromyces, including seven new species. These new species were studied in detail using a polyphasic approach combining phenotypic, molecular and extrolite data. The four new Penicillium species belong to sections Sclerotiora (Penicillium fernandesiae sp. nov., Penicillium mellis sp. nov., Penicillium meliponae sp. nov.) and Gracilenta (Penicillium apimei sp. nov.) and the three new Talaromyces species to sections Helici (Talaromyces pigmentosus sp. nov.), Talaromyces (Talaromyces mycothecae sp. nov.) and Trachyspermi (Talaromyces brasiliensis sp. nov.). The invalidly described species Penicillium echinulonalgiovense sp. nov. was also isolated during the survey and this species is validated here.

Keywords: 8 new taxa; Aspergillaceae; Fungal ecology; Polyphasic approach; Taxonomy; Trichocomaceae.

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Conflict of interest statement

All authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Phylogeny based on the combined ITS, BenA, CaM and RPB2 data set for species classified in Penicillium section Gracilenta. Penicillium abidjanum CBS 246.67 was chosen as outgroup
Fig. 2
Fig. 2
Phylogeny based on the combined ITS, BenA and CaM data set for species classified in Penicillium section Lanata-Divaricata. Penicillium glabrum CBS 125543 was chosen as outgroup
Fig. 3
Fig. 3
Phylogeny based on the combined ITS, BenA, and CaM data set for species classified in Penicillium section Sclerotiora. Penicillium glabrum CBS 125543 was chosen as outgroup
Fig. 4
Fig. 4
Phylogeny based on the combined ITS, BenA, CaM and RPB2 data set for species classified in Talaromyces section Helici. Talaromyces ucrainicus CBS 162.67 was chosen as outgroup
Fig. 5
Fig. 5
Phylogeny based on the combined ITS, BenA, CaM and RPB2 data set for species classified in Talaromyces section Talaromyces. Talaromyces dendriticus CBS 660.80 was chosen as outgroup
Fig. 6
Fig. 6
Phylogeny based on the combined ITS, BenA, CaM and RPB2 data set for species classified in Talaromyces section Trachyspermi. Talaromyces purpurogenus CBS 286.36 was chosen as outgroup
Fig. 7
Fig. 7
Morphological characters of Penicillium apimei CBS 142502. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia. e–h Conidiophores. Scale bars 10 μm
Fig. 8
Fig. 8
Morphological characters of Penicillium fernandesiae CBS 142500. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia e–i Conidiophores. Scale bars 10 μm
Fig. 9
Fig. 9
Morphological characters of Penicillium meliponae CBS 142495. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia. e–i Conidiophores. Scale bars 10 μm
Fig. 10
Fig. 10
Morphological characters of Penicillium mellis CBS 142499. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia. e Sclerotia. f–j Conidiophores. Scale bars 10 μm
Fig. 11
Fig. 11
Morphological characters of Talaromyces brasiliensis CBS 142493. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia. e–h Conidiophores. Scale bars 10 μm
Fig. 12
Fig. 12
Morphological characters of Talaromyces mycothecae CBS 142494. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia. e–h Conidiophores. Scale bars 10 μm
Fig. 13
Fig. 13
Morphological characters of Talaromyces pigmentosus CBS 142805. a Colonies from left to right (top row) MEA, CYA, YES and OA; (bottom row) CYA reverse, MEA reverse, YES reverse and CREA. b Texture on CYA. c Texture on MEA. d Conidia. e–h Conidiophores. Scale bars 10 μm
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