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. 2018 Feb;11(2):227-230.
doi: 10.14202/vetworld.2018.227-230. Epub 2018 Feb 21.

Interspecies comparison of probiotics isolated from different animals

Amr M Abdou  1 , Riham H Hedia  1 , Shimaa T Omara  1 , Mohamed Abd El-Fatah Mahmoud  2 , Mai M Kandil  1 , M A Bakry  1
Affiliations

Interspecies comparison of probiotics isolated from different animals

Amr M Abdou et al. Vet World. 2018 Feb.

Abstract

Aim: The aim of the current study was to isolate and identify naturally occurring probiotic Lactobacillus species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in probiotics on the species level.

Materials and methods: A total of 44 fecal and milk samples were collected under aseptic conditions from cattle, buffalo, camel, sheep, goats, and fish. The samples were cultured, and the isolated strains were confirmed biochemically and molecularly using 16S rRNA multiplex polymerase chain reaction (PCR) analysis following DNA extraction from the bacterial isolates.

Results: A total of 31 isolates identified as lactobacilli were isolated from cattle milk, goat feces, sheep feces, fish feces, buffalo milk, camel milk, and goats' milk. Lactobacillus species were identified based on the size of the PCR product. The results showed that different species were different in their lactobacilli content. At the same time, there were some differences between individuals of the same species.

Conclusion: The diversity of probiotic strains isolated from different animal species implies different types of benefits to the host. Although it would be both money - and time-consuming research, discovering the benefit of each of these strains may provide very important information for the health of both human and animal. Furthermore, transferring these beneficial effects either to individuals within the same species or between different species would be of great importance.

Keywords: Lactobacillus; multiplex polymerase chain reaction; probiotics.

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Figures

Figure-1
Figure-1
Agarose gel electrophoreses of polymerase chain reaction (PCR) products from multiplex PCR assays. Multiplex PCR assays were performed with a mixture of seven species-specific or group-specific primers for L. acidophilus, L. bulgaricus (same as L. delbrueckii subsp. bulgaricus), L. casei-group L. gasseri, L. plantarum, L. reuteri, and L. rhamnosus and two bacterial conserved primers. Lanes 1-12 designate the PCR product from each genomic DNA extracted from single or mixed cell suspension isolated from representative host used as PCR template. Lane 1: L. casei, L. gasseri; Lane 2: L. casei, L. acidophilus, L. delbrueckii; Lane 3: L. acidophilus, L. rhamnosus, L. plantarum, L. gasseri, L. delbrueckii; Lane 4: L. acidophilus; Lane 5: L. rhamnosus, L. plantarum, L. gasseri, L. delbrueckii; Lane 6: L. casei; Lane 7: Negative control; Lane 8: L. casei, L. acidophilus, L. delbrueckii; Lane 9: L. acidophilus, L. rhamnosus, L. gasseri, L. delbrueckii; Lane 10: L. acidophilus, L. rhamnosus, L. gasseri, L. delbrueckii; Lane 11: L. acidophilus, Lane 12: L. acidophilus, L. rhamnosus, L. plantarum; Lane M, 100 bp-DNA ladder.
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