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. 2018 Jan-Mar;12(1):36-42.
doi: 10.4103/ejd.ejd_54_17.

Comparison of osteoclastogenesis and local invasiveness of ameloblastoma and keratocystic odontogenic tumor

Natheer H Al-Rawi  1 , Ammar K Al-Siraj  2 , Ahlam H Majeed  2
Affiliations

Comparison of osteoclastogenesis and local invasiveness of ameloblastoma and keratocystic odontogenic tumor

Natheer H Al-Rawi et al. Eur J Dent. 2018 Jan-Mar.

Abstract

Objectives: The aim of this study was to compare the expression of receptor-activated nuclear factor kappa B (RANK) with its ligand (RANKL) and matrix metalloproteinase-2 (MMP2) in solid/multicystic ameloblastomas (ABs) and keratocystic odontogenic tumors (KOTs).

Materials and methods: The expression of MMP2, RANK, and RANKL molecules was evaluated in 13 ABs and 14 KOTs by immunohistochemistry. The expressions were calculated in the odontogenic epithelial cells as well as the stromal cells.

Results: Odontogenic epithelia of AB expressed MMP2, RANK, and RANKL significantly higher than that of KOTs (P < 0.05). The expression of MMP2, RANK, and RANKL was highest in plexiform subtype (79.9%, 81.08%, and 65.1%, respectively). KOTs without daughter epithelia nests expressed both MMP2 and RANK the least (56.06% and 47.5%, respectively). Stromal cells, on the other hand, expressed similar MMP2 pattern in odontogenic epithelia of both AB and KOT. RANKL was expressed weaker in the stromal cells of both lesions.

Conclusion: Invasive biological and osteolytic behaviors of both lesions were evaluated in this study. It was found to be more in AB than keratocystic odontogenic. A significant expression of MMP2, RANK, and RANKL in both KOTs associated with microcyst and plexiform type AB as well.

Keywords: Keratocystic odontogenic tumor; matrix metalloproteinase-2; odontogenic keratocysts; receptor-activated nuclear factor kappa B; receptor-activated nuclear factor kappa B ligand; solid/multicystic ameloblastoma.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
(a-d) Matrix metalloproteinase-2 expression in odontogenic epithelium, follicular ameloblastoma, plexiform ameloblastoma, keratocystic odontogenic tumor (−dentigerous cysts), keratocystic odontogenic tumor (+dentigerous cysts)
Figure 2
Figure 2
(a-d) Receptor-activated nuclear factor kappa B expression in odontogenic epithelium, follicular ameloblastoma, plexiform ameloblastoma, keratocystic odontogenic tumor (−dentigerous cysts), keratocystic odontogenic tumor (+dentigerous cysts)
Figure 3
Figure 3
(a-d) Receptor-activated nuclear factor kappa B ligand expression in odontogenic epithelium, follicular ameloblastoma, plexiform ameloblastoma, keratocystic odontogenic tumor
See this image and copyright information in PMC

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