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. 2018 Mar 29;12(2):024111.
doi: 10.1063/1.5021179. eCollection 2018 Mar.

Detection of viability of micro-algae cells by optofluidic hologram pattern

Junsheng Wang  1 , Xiaomei Yu  1 , Yanjuan Wang  1 , Xinxiang Pan  2 , Dongqing Li  3
Affiliations

Detection of viability of micro-algae cells by optofluidic hologram pattern

Junsheng Wang et al. Biomicrofluidics. .

Abstract

A rapid detection of micro-algae activity is critical for analysis of ship ballast water. A new method for detecting micro-algae activity based on lens-free optofluidic holographic imaging is presented in this paper. A compact lens-free optofluidic holographic imaging device was developed. This device is mainly composed of a light source, a small through-hole, a light propagation module, a microfluidic chip, and an image acquisition and processing module. The excited light from the light source passes through a small hole to reach the surface of the micro-algae cells in the microfluidic chip, and a holographic image is formed by the diffraction light of surface of micro-algae cells. The relation between the characteristics in the hologram pattern and the activity of micro-algae cells was investigated by using this device. The characteristics of the hologram pattern were extracted to represent the activity of micro-algae cells. To demonstrate the accuracy of the presented method and device, four species of micro-algae cells were employed as the test samples and the comparison experiments between the alive and dead cells of four species of micro-algae were conducted. The results show that the developed method and device can determine live/dead microalgae cells accurately.

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Figures

FIG. 1.
FIG. 1.
(a) Schematic of hologram pattern formation in our developed system; (b) schematic of relation module between the refractive index and the hologram pattern of micro-algae cells.
FIG. 2.
FIG. 2.
Schematic of lens-free optofluidic holographic imaging system.
FIG. 3.
FIG. 3.
Structure diagram of the designed microfluidic chip.
FIG. 4.
FIG. 4.
(a) The holographic images of the living micro-algae cells (Platymonas subcordiformis). (b) The holographic images of the dead micro-algae cells (Platymonas subcordiformis).
FIG. 5.
FIG. 5.
Parameters analysis of hologram pattern of the micro-algae cells: (a) Pyramimonas sp., (b) Closterium lunula, and (c) Isotypic sp.
FIG. 6.
FIG. 6.
Judgment of liveness and death of the micro-algae cells by Dgray in hologram pattern.
See this image and copyright information in PMC

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