Isolation of Oropouche Virus from Febrile Patient, Ecuador
- PMID: 29664378
- PMCID: PMC5938787
- DOI: 10.3201/eid2405.171569
Isolation of Oropouche Virus from Febrile Patient, Ecuador
Abstract
We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.
Keywords: Arbovirus; Bunyaviridae; Ecuador; Oropouche; Orthobunyaviridae; South America; bunyavirus; communicable disease; high-throughput nucleotide sequencing; metagenomic; orthobunyavirus; virus; viruses.
References
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- Manock SR, Jacobsen KH, de Bravo NB, Russell KL, Negrete M, Olson JG, et al. Etiology of acute undifferentiated febrile illness in the Amazon basin of Ecuador. Am J Trop Med Hyg. 2009;81:146–51. - PubMed
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