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. 2018 Apr 17;8(1):6075.
doi: 10.1038/s41598-018-24443-7.

Insight into the cold adaptation and hemicellulose utilization of Cladosporium neopsychrotolerans from genome analysis and biochemical characterization

Rui Ma  1   2 , Huoqing Huang  1 , Yingguo Bai  1 , Huiying Luo  1 , Yunliu Fan  2 , Bin Yao  3
Affiliations

Insight into the cold adaptation and hemicellulose utilization of Cladosporium neopsychrotolerans from genome analysis and biochemical characterization

Rui Ma et al. Sci Rep. .

Abstract

The occurrence of Cladosporium in cold ecosystems has been evidenced long before, and most of the knowledge about nutrient utilization of this genus is sporadic. An alpine soil isolate C. neopsychrotolerans SL-16, showing great cold tolerance and significant lignocellulose-degrading capability, was sequenced to form a 35.9 Mb genome that contains 13,456 predicted genes. Functional annotation on predicted genes revealed a wide array of proteins involved in the transport and metabolism of carbohydrate, protein and lipid. Large numbers of transmembrane proteins (967) and CAZymes (571) were identified, and those related to hemicellulose degradation was the most abundant. To undermine the hemicellulose (xyaln as the main component) utilization mechanism of SL-16, the mRNA levels of 23 xylanolytic enzymes were quantified, and representatives of three glycoside hydrolase families were functionally characterized. The enzymes showed similar neutral, cold active and thermolabile properties and synergistic action on xylan degradation (the synergy degree up to 15.32). Kinetic analysis and sequence and structure comparison with mesophilic and thermophilic homologues indicated that these cold-active enzymes employed different cold adaptation strategies to function well in cold environment. These similar and complementary advantages in cold adaptation and catalysis might explain the high efficiency of lignocellulose conversion observed in SL-16 under low temperatures.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Lignocellulose-degrading activities of C. neopsychrotolerans SL-16 after 7-day-growth at different temperatures.
Figure 2
Figure 2
KOG (a), GO (b) and KEGG (c) classification of predicted genes in C. neopsychrotolerans SL-16.
Figure 3
Figure 3
Biochemical characterization of purified recombinant Xyn10A, Xyn101A and Xyl43A from C. neopsychrotolerans SL-16. (A) pH-activity profiles. (B) Temperature-activity profiles. (C) pH-stability profiles. (D) Temperature-stability profiles. Each value in the panel represents the mean ± SD (n = 3).
See this image and copyright information in PMC

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