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. 1988 Apr 11;16(7):2873-84.
doi: 10.1093/nar/16.7.2873.

A new and fast method for preparing high quality lambda DNA suitable for sequencing

G Manfioletti  1 , C Schneider
Affiliations
Free PMC article

A new and fast method for preparing high quality lambda DNA suitable for sequencing

G Manfioletti et al. Nucleic Acids Res. .
Free PMC article

Abstract

A method is described for the rapid purification of high quality lambda DNA. The method can be used from either liquid or plate lysates and on a small scale or a large scale. It relies on the preadsobtion of all polyanions present in the lysate to an "insoluble" anion-exchange matrix (DEAE or TEAE). Phage particles are then disrupted by combined treatment with EDTA/proteinase K and the resulting DNA is precipitated by the addition of the cationic detergent cetyl (or hexadecyl)-trimethyl ammonium bromide-CTAB ("soluble" anion-exchange matrix). The precipitated CTAB-DNA complex is then exchanged to Na-DNA and ethanol precipitated. The resultant purified DNA is suitable for enzymatic reactions and provides a high quality template for dideoxy-sequence analysis.

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References

    1. Nucleic Acids Res. 1975 Jan;2(1):61-78 - PubMed
    1. Biochemistry. 1977 Oct 18;16(21):4743-51 - PubMed
    1. Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 - PubMed
    1. Nature. 1963 Jul 20;199:280-2 - PubMed
    1. DNA. 1985 Feb;4(1):39-49 - PubMed

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