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. 2018 Jun 1;2018(6).
doi: 10.1101/pdb.prot094524.

In Vitro Fertilization in Mice

Toru Takeo, Naomi Nakagata

In Vitro Fertilization in Mice

Toru Takeo et al. Cold Spring Harb Protoc. .

Abstract

The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).

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