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. 2018 Feb 19:2018:3142073.
doi: 10.1155/2018/3142073. eCollection 2018.

In Vitro Wound Healing Potential of Stem Extract of Alternanthera sessilis

Katyakyini Muniandy  1 , Sivapragasam Gothai  1 , Woan Sean Tan  1 , S Suresh Kumar  2 , Norhaizan Mohd Esa  3 , Govindasamy Chandramohan  4 , Khalid S Al-Numair  4 , Palanisamy Arulselvan  1   5
Affiliations

In Vitro Wound Healing Potential of Stem Extract of Alternanthera sessilis

Katyakyini Muniandy et al. Evid Based Complement Alternat Med. .

Erratum in

Abstract

Impaired wound healing is one of the serious problems among the diabetic patients. Currently, available treatments are limited due to side effects and cost effectiveness. In line with that, we attempted to use a natural source to study its potential towards the wound healing process. Therefore, Alternanthera sessilis (A. sessilis), an edible and medicinal plant, was chosen as the target sample for the study. During this investigation, the wound closure properties using stem extract of A. sessilis were analyzed. Accordingly, we analyzed the extract on free radical scavenging capacity and the cell migration of two most prominent cell types on the skin, human dermal fibroblast (NHDF), keratinocytes (HaCaT), and diabetic human dermal fibroblast (HDF-D) to mimic the wound healing in diabetic patients. The bioactive compounds were identified using gas chromatography-mass spectrometry (GC-MS). We discovered that the analysis exhibited a remarkable antioxidant, proliferative, and migratory rate in NHDF, HaCaT, and HDF-D in dose-dependent manner, which supports wound healing process, due to the presence of wound healing associated phytocompounds such as Hexadecanoic acid. This study suggested that the stem extract of A. sessilis might be a potential therapeutic agent for skin wound healing, supporting its traditional medicinal uses.

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Figures

Figure 1
Figure 1
The DPPH scavenging action of stem extract of A. sessilis increased in a concentration-dependent manner. Values are mean ± SD (n = 3).
Figure 2
Figure 2
Effect of ethanolic extract of A. sessilis stem on viability of NHDF (a), HaCaT (b), and HDF-D (c). Cell viability following incubation with indicated concentrations of crude extract for 24 h was determined using the MTT assay. Cell viability is expressed as a percentage of untreated cells. Results shown in the graphs are mean ± SD obtained from triplicate experiments.
Figure 3
Figure 3
The migration rate in percentage for NHDF (a), HaCaT (b), and HDF-D (c) after treatment with stem extract of A. sessilis for 24 hrs. Quantitative analysis of the migration rate was analyzed with the use of ImageJ software. Data are expressed as mean ± standard deviation from three individual experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus control group.
Figure 4
Figure 4
In vitro scratch assay (×40 magnification). NHDF (a), HaCaT (b), and HDF-D (c) cells were scratched and treated with and without treatment of varying concentrations of plant extract. Ethanolic extract of stem part of A. sessilis showed positive cell proliferation and cell migration as compared with control group (without treatment).
Figure 5
Figure 5
Gas chromatogram for stem extract of A. sessilis.
Figure 6
Figure 6
See this image and copyright information in PMC

References

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