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. 2018 Jan 1;10(1):e1-e6.
doi: 10.4317/jced.54471. eCollection 2018 Jan.

Sealing effectiveness of fissure sealant bonded with universal adhesive systems on saliva-contaminated and noncontaminated enamel

Mahtab Memarpour  1 , Fereshteh Shafiei  2 , Mehran Zarean  3 , Faranak Razmjoei  4
Affiliations

Sealing effectiveness of fissure sealant bonded with universal adhesive systems on saliva-contaminated and noncontaminated enamel

Mahtab Memarpour et al. J Clin Exp Dent. .

Abstract

Background: The effectiveness of sealants is dependent upon their adhesion to enamel surface. The aim of the study was to evaluate the sealing ability of a pit and fissure sealant used with a universal adhesive (etch-and-rinse vs. self-etch modes) when the site is contaminated with saliva. Adhesive properties were evaluated as microleakage and scanning electron microscopic (SEM) characteristics.

Material and methods: A total of 72 mandibular third molars were randomly divided into 6 groups (n=12). Occlusal pits and fissures were sealed with an unfilled resin fissure sealant (FS) material with or without saliva contamination. The groups included: 1) phosphoric acid etching + FS (control), 2) phosphoric acid etching + Scotchbond Universal (etch-and-rinse) + FS, 3) phosphoric acid etching + saliva + Scotchbond Universal (etch-and-rinse) + FS, 4) Scotchbond Universal (self-etching) + FS,5) Scotchbond Universal (self-etching) + saliva + FS, and 6) Scotchbond Universal (self-etching) + saliva + Scotchbond Universal + FS. After thermocycling, the teeth were placed in 0.5% fuchsin, sectioned, and evaluated by digital microscopy. Two samples from each group were also observed by SEM. The data were analyzed with Kruskal-Wallis and Mann-Whitney tests for a significance of p<0.05.

Results: There were significant differences among groups. Groups 1,2 and 4 showed the least microleakage, with no significant differences among groups. Saliva contamination led to increased microleakage and gap formation in SEM images in groups 3, 5 and 6.

Conclusions: The fissure sealing ability of the universal adhesive in etch-and-rinse or self-etch modes was similar to that of conventional acid etching. Saliva contamination had a negative effect on sealant adhesion to pretreated enamel. Key words:Pit and fissure sealant, Universal adhesive, Saliva.

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Conflict of interest statement

Conflict of interest statement: The authors state that they have no conflicts of interest.

Figures

Figure 1
Figure 1
SEM image of the tooth–fissure sealant interface when SBU was applied in etch-and-rinse mode.
Figure 2
Figure 2
SEM image of the tooth–fissure sealant interface when SBU was applied in self-etch mode.
Figure 3
Figure 3
SEM image of the tooth–fissure sealant interface when SBU was used in the presence of saliva contamination.
See this image and copyright information in PMC

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