Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan 1;10(1):e1-e6.
doi: 10.4317/jced.54471. eCollection 2018 Jan.

Sealing effectiveness of fissure sealant bonded with universal adhesive systems on saliva-contaminated and noncontaminated enamel

Mahtab Memarpour  1 , Fereshteh Shafiei  2 , Mehran Zarean  3 , Faranak Razmjoei  4
Affiliations

Sealing effectiveness of fissure sealant bonded with universal adhesive systems on saliva-contaminated and noncontaminated enamel

Mahtab Memarpour et al. J Clin Exp Dent. .

Abstract

Background: The effectiveness of sealants is dependent upon their adhesion to enamel surface. The aim of the study was to evaluate the sealing ability of a pit and fissure sealant used with a universal adhesive (etch-and-rinse vs. self-etch modes) when the site is contaminated with saliva. Adhesive properties were evaluated as microleakage and scanning electron microscopic (SEM) characteristics.

Material and methods: A total of 72 mandibular third molars were randomly divided into 6 groups (n=12). Occlusal pits and fissures were sealed with an unfilled resin fissure sealant (FS) material with or without saliva contamination. The groups included: 1) phosphoric acid etching + FS (control), 2) phosphoric acid etching + Scotchbond Universal (etch-and-rinse) + FS, 3) phosphoric acid etching + saliva + Scotchbond Universal (etch-and-rinse) + FS, 4) Scotchbond Universal (self-etching) + FS,5) Scotchbond Universal (self-etching) + saliva + FS, and 6) Scotchbond Universal (self-etching) + saliva + Scotchbond Universal + FS. After thermocycling, the teeth were placed in 0.5% fuchsin, sectioned, and evaluated by digital microscopy. Two samples from each group were also observed by SEM. The data were analyzed with Kruskal-Wallis and Mann-Whitney tests for a significance of p<0.05.

Results: There were significant differences among groups. Groups 1,2 and 4 showed the least microleakage, with no significant differences among groups. Saliva contamination led to increased microleakage and gap formation in SEM images in groups 3, 5 and 6.

Conclusions: The fissure sealing ability of the universal adhesive in etch-and-rinse or self-etch modes was similar to that of conventional acid etching. Saliva contamination had a negative effect on sealant adhesion to pretreated enamel. Key words:Pit and fissure sealant, Universal adhesive, Saliva.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest statement: The authors state that they have no conflicts of interest.

Figures

Figure 1
Figure 1
SEM image of the tooth–fissure sealant interface when SBU was applied in etch-and-rinse mode.
Figure 2
Figure 2
SEM image of the tooth–fissure sealant interface when SBU was applied in self-etch mode.
Figure 3
Figure 3
SEM image of the tooth–fissure sealant interface when SBU was used in the presence of saliva contamination.
See this image and copyright information in PMC

References

    1. American Academy of Pediatric Dentistry and American Dental Association. Evidence-based clinical practice guideline for the use of pit-and-fissure sealants. Pediatr Dent. 2016;38:263–79. - PubMed
    1. Memarpour M, Shafiei F, Shokouh P, Shaddel M. Evaluation of a school-based pit and fissure sealant programme in Iranian children. Oral Health Prev Dent. 2011;9:381–6. - PubMed
    1. Simonsen RJ. From prevention to therapy: minimal intervention with sealants and resin restorative materials. J Dent. 2011;39 Suppl 2:S27–33. - PubMed
    1. Erbas Unverdi G, Atac SA, Cehreli ZC. Effectiveness of pit and fissure sealants bonded with different adhesive systems: a prospective randomized controlled trial. Clin Oral Investig. 2017;21:2235–2243. - PubMed
    1. Meller C, Reichenmiller K, Schwahn C, Samietz S, Blunck U. Resin-based pit-and-fissure sealants: microleakage reduction and infiltration enhancement using a bonding agent. J Adhes Dent. 2015;17:59–65. - PubMed

LinkOut - more resources