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. 2018 Jan 1;10(1):e7-e13.
doi: 10.4317/jced.53957. eCollection 2018 Jan.

Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources

Iman M Aboushady  1 , Zeinab A Salem  1 , Dina Sabry  2 , Abbas Mohamed  3
Affiliations

Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources

Iman M Aboushady et al. J Clin Exp Dent. .

Abstract

Background: Mesenchymal stem cells (MSCs) can regenerate missing tissues and treat diseases. Hence, the current work aimed to compare the proliferation rate and the osteogenic differentiation potential of bone marrow MSCs (BMSCs), gingival MSCs (GMSCs) and submandibular MSCs (SMSCs).

Material and methods: MSCs derived from bone marrow, gingiva and submandibular salivary gland were isolated and cultured from rats. The proliferation capacity was judged by MTT proliferation Assay. Osteogenic differentiation was assessed by Alzarin red stain and quantitative RT-PCR was performed for Runx-2 and MMP-13.

Results: The highest significant proliferation was estimated in the BMSCs compared to GMSCs and SMSCs (p-value was < 0.01). All studied cell types formed mineralized nodules as stained with Alizarin Red stain at the 3rd passage of differentiation. However, BMSCs seemed to generate the highest level of mineralization compared to GMSCs and SMSCs. RT-PCR revealed that the expression of Runx-2 and MMP-13 mRNAs was significantly increased in the BMSCs compared to GMSCs and SMSCs (p-value was < 0.01).

Conclusions: BMSCs displayed maximum osteogenesis results followed by the GMSCs and lastly by the SGSCs. Thus, it could be recommended that GMSCs can be used as a second choice after BMSCs when bone tissue reconstruction is needed. Key words:Mesenchymal stem cells, osteogenic differentiation, Runx-2, MMP-13.

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Conflict of interest statement

Conflict of interest statement: The authors have declared that no conflict of interest exist.

Figures

Figure 1
Figure 1
BM-MSCs at 3rd passage differentiated into osteoblasts and stained with Alzarin red. (arrows showed higher calcium mineralization deposits compared to other groups).
Figure 2
Figure 2
GMSCs at 3rd passage differentiated into osteoblasts and stained with Alzarin red. (arrows indicating moderate calcium mineralization deposits).
Figure 3
Figure 3
SMSCs at 3rd passage differentiated into osteoblasts and stained with Alzarin red. (arrows indicating lower calcium mineralization deposits).
See this image and copyright information in PMC

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