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. 2018 Feb 19;8(4):e00941.
doi: 10.1002/brb3.941. eCollection 2018 Apr.

Identification of aberrant circulating miRNAs in Parkinson's disease plasma samples

Lei Chen  1   2 , Junxiu Yang  3 , Jinhui Lü  4 , Shanshan Cao  1   2 , Qian Zhao  4 , Zuoren Yu  4
Affiliations

Identification of aberrant circulating miRNAs in Parkinson's disease plasma samples

Lei Chen et al. Brain Behav. .

Abstract

Objective: To detect the aberrant expression of circulating miRNAs and explore the potential early diagnostic biomarkers in patients with Parkinson's disease (PD).

Methods: Plasma samples were collected from 25 treatment-naïve PD-diagnosed patients and 25 healthy controls followed by a real-time PCR-based miRNA screening analysis of neuron disease-related miRNAs.

Results: A subset of miRNAs with aberrant expression levels in the plasma of PD-diagnosed patients were identified including upregulation of miR-27a and downregulation of let-7a, let-7f, miR-142-3p, and miR-222 with the AUC values more than 0.8 derived from the receiver operating characteristic curves.

Conclusions: The high sensitivity and specificity of the circulating miRNAs may enable early diagnosis of PD. The study provides a group of novel miRNA candidates for detecting PD.

Keywords: Parkinson's disease; circulating miRNAs; diagnosis.

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Figures

Figure 1
Figure 1
Real‐time PCR analyses of plasma samples from PD‐diagnosed patients and normal controls using the PD‐miRNA panel. (a) The RNA analysis using the Agilent 2100 bioanalyzer of the representative control sample. (b) The RNA analysis using the Agilent 2100 bioanalyzer of the representative Parkinson's disease (PD) sample. (c) The amplification curves of real‐time PCR analysis on miRNAs in the representative control sample. (d) The amplification curves of real‐time PCR on miRNAs in the representative PD sample. (e) Scatter plot showing the Ct (cycle threshold) values of miR‐16 at location A3 in the panel was perfectly repeated by that at location H10 in all 50 samples, forming a very good linear correlation with R 2 = .9697, demonstrating the reproducibility of the miRNA analysis by real‐time PCR
Figure 2
Figure 2
miR‐27a showed upregulation in the plasma of PD‐diagnosed patients compared with normal controls. (a) Scatter plot showing the expression levels of circulating miR‐27a in Parkinson's disease (PD) and control samples. p = .0269. (b) Receiver operating characteristic curves (ROC) of miR‐27a were generated using MedCalc Software, with the AUC value 0.803
Figure 3
Figure 3
Scatter plot showing the expression levels of indicated miRNAs in all the tested samples. These miRNAs showed at least two folds less in the plasma of PD‐diagnosed patients than healthy controls, and adjusted p values ≤.05. The Mann–Whitney test was used for statistical analysis using IBM SPSS Statistics 21.0 software
Figure 4
Figure 4
Receiver operating characteristic curves (ROC) of all 14 downregulated miRNAs, including let‐7a, let‐7f, miR‐142‐3p, and miR‐222 with the AUC values more than 0.8
See this image and copyright information in PMC

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